DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20160101

Evaluation of analgesic activity of Ficus racemosa leaf extract using acetic acid induced writhing method in mice

Niket Rai, Savita Vyas, Pradeep Phadnis

Abstract


Background: To evaluate the analgesic activity of Ficus racemosa leaf extract and its comparison with standard drug using acetic acid induced writhing method in mice.

Methods: Chloroform extract of leaves was obtained using soxhlet apparatus. Isolated fraction of extract (FRE) was used for the experiments. Swiss albino mice of either sex, average weight 20-25 gms were used for experiments. Animals were divided into 6 groups consisting of 6 animals each.1% acetic acid was administered intra peritoneally to the experimental animals to create pain sensation. Each group received particular treatment 30 minutes prior to administration of acetic acid. Each mouse of all groups were observed individually for counting number of writhes they made in 15 minutes commencing just 5 minutes after the administration of acetic acid. Diclofenac was taken as standard drug.

Results: FRE at the dose of 50 mg/kg did not shows any significant analgesic activity while at the dose of 100 and 200 mg/kg it showed significant analgesic activity as compare to control group. FRE at dose of 200 mg/kg showed significant analgesic activity as compare to diclofenac in writhing model. Diclofenac in combination with 50 mg/kg dose of FRE produced significant analgesic activity when compared to control value or either treatment alone.

Conclusions: FRE endowed with peripheral analgesic properties in dose dependent manner. And also enhances the analgesic effect of diclofenac. However further study is needed in order to understand the precise mechanism.


Keywords


Ficus racemosa, Analgesics, Diclofenac sodium, Albino mice

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References


Kalgutkar AS, Marnett AB, Crews BC, Remmel RP, Marnett LJ. Ester and amide derivatives of the non-steroidal anti-inflammatory drug, indomethacin, as selectivecyclooxygenase-2 inhibitors. J Med Chem. 2000;43(15):2860-70.

Anonymous. The wealth of india. Council of scientific and industrial research, New Delhi, India. 1952:35-6.

Mandal SC, Tapan K, Maity J, Das M, Pal M, Saha BP. Hepatoprotective activity of Ficus racemosa leaf extract on liver damage caused by carbon tetrachloride in rats. Phytother. Res. 1999;13(5):430-2.

Khan N, Sultana S. Chemomodulatory effect of Ficus racemosa extract against chemically induced renal carcinogenesis and oxidative damage response in Wistar rats. Life Sci. 2005;29:1194-210.

Rao BR, Murugesan T, Sinha S, Saha BP, Pal M, Mandal SC. Glucose lowering efficacy of Ficus racemosa bark extract in normal and alloxan diabetic rats. Phytother Res. 2002;16:590-2.

Mandal SC, Saha BP and Pal M. studies on bacterial activity of Ficus racemosa leaf extract. Phytother Res. 2000;14(4):278-80.

Rao BR, Anipama K, Swaroop A, Murugesan T, Pal M, Mandal SC. Evaluation of anti-pyretic potential of Ficus racemosa bark. Phytomedicine. 2002;9:731-3.

Rao BR, Murugesan T, Pal M, Saha BP, Mandal SC. Antitussive potential of methanol extract of stem bark of Ficus racemosa Linn. Phytother Res. 2003;17:1117-8.

Ratnasooriya WD, Jayakody JR, Nadarajah T. Antidiuretic activity of aqueous bark extract of Sri Lankan Ficus racemosa in rats. Acta Biol Hung. 2003;54(3-4):357-63.

Kokate CK. Textbook of practical pharmacognosy, 4th ed. Vallabh Prakashan, New Delhi. 2003:143.

Ghani A. Medicinal plants of Bangladesh. The Asiatic society of Bangladesh. Dhaka, Bangladesh. 2003:500-4.

Organization for economic cooperation and development. OECD guidelines for the testing of chemicals. OECD guideline 420. Acute oral toxicity: up-and-down procedure, 2001.

Joseph B, Raj SJ. Phytopharmacological properties of Ficus Racemosa Linn-: an overview. Int J Pharm Sci Rev Res. 2010;3(2):134-8.

Hasan SMR, Hossain MM, Akter R, Jamila M, Mazumder MEH, Alam MA, et al. Analgesic activity of the different fractions of the aerial parts of Commelina benghalensis Linn. Int J Pharmacol. 2010;6(1):63-7.

Ahmed F, Hossain MH, Rahman AA, Shahid IZ. Antinociceptive and sedative effects of the bark of Cerbera odollam Gaertn. Orient Pharm Experimental Med. 2006;6:344-8.

Duarte IDG, Nakamura M, Ferreira SH. Participation of the sympathetic system in acetic acid-induced writhing in mice. Brazilian J Med Biol Res. 1998;21:341-3.

Derardt R, Jougney S, Delevalccee F, Falhout M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. European J Pharmacol. 1980;51:17-24.

Zakaria ZA, Abdul Gani ZDF. Antinociceptive, anti-inflammatory, and antipyretic properties of an aqueous extract of Dicranopteris linearis leaves in experimental animal models. J Natur Med. 2008;62:179-87.

Ferdous M, Rouf R, Shilpi JA, Uddin SJ. Antinociceptive activity of the ethanolic extract of Ficus racemosa Linn. (Moraceae). Orient Pharm Experiment Med. 2008;8:93-6.

Collier HOJ, Dinneen LC, Johnson CA, Schneide C, 1968. Abdominal constriction response and its suppression by analgesic drugs in mouse. British J Pharmacol. 1968;32:265-8.

Toma W, Graciosa JS, Hiruma-Lima CA, Andrade FDP, Vilegas W, Souza Brita ARM, 2003. Evaluation of the analgesic and antiedematogenic activities of Quassia amara bark extract. J Ethnopharmacol. 2003;85:19-23.

Rajnarayana K, Reddy MS, Chaluvadi MR, Krishna DR. Biflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol. 2001;33:2-16.

Rao MR, Rao YM, Rao AV, Prabhkar MC, Rao CS, Muralidhar N. Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuate. J Ethnopharmacol. 1998;62:63-6.

Vanu MR, Palanivelu S, Panchanatham S. Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium Linn. Nut milk extract in experimental inflammatory conditions. Biol Pharm Bull. 2006;29:693-700.

Uche FI, Aprioku JS. The phytochemical constituents, analgesic and anti-inflammatory effects of methanol extract of Jatropha curcas leaves in mice and Wister albino rats. J Appl Sci Environ Manag. 2008;12(4):99-102.