The wound healing property of ethanolic extract of Michelia champaca flowers in diabetic rats


  • Amoolya Gowda Department of Pharmacology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
  • Venkatesh Shanbhag Department of Surgery, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India
  • Eesha Rao Bangalore Department of Pharmacology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
  • Smita Shenoy Department of Pharmacology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
  • Krishnananda Prabhu Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
  • Mukunda Narayanareddy Department of Pharmacology, Ramaiah Medical College, Bengaluru, Karnataka, India
  • Tara Shanbhag Department of Pharmacology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India


Incision wound, Excision wound, Hydroxyproline and diabetic rats


Background: The plant Michelia champaca (MC) is widely used in the treatment of inflammation, constipation, dysmenorrhea, ulcers, wounds, fever, and cough. The aim was to evaluate the wound healing property of ethanolic extract of MCflowers in streptozotocin-induced diabetes in rats.

Methods: Wound healing activity was assessed by incision and excision wound models. Five groups of n=6 rats and n=14 rats were used for incision and excision wound models, respectively. Group I rats, non-diabetic control and Group II rats diabetic control, received 1 ml of 0.5% caboxymethylcellolose, which was used to prepare a suspension of ethanolic extracts of MC. Group III, IV and V rats were given MC extract the suspension of 100 mg/kg, 200 mg/kg and 300 mg/kg respectively. Parameters observed were breaking strength of incision wound and wound contraction, epithelialization, hydroxyproline content of excision wound respectively. Results were analyzed using one-way analysis of variance, followed by Tukey’s post-hoc test.

Results: Breaking strength, rate of wound contraction and hydroxyproline content were significantly increased, and the period of epithelialization was significantly reduced in Group IV and V rats respectively.

Conclusion: Oral administration of ethanolic extract of MC promotes wound healing in diabetic rats. Hence, further study in humans is suggested.


Robson MC. Wound infection. A failure of wound healing caused by an imbalance of bacteria. Surg Clin North Am. 1997;77(3):637-50.

Snyder RJ. Treatment of nonhealing ulcers with allografts. Clin Dermatol. 2005;23:388-95.

Varier PS. Indian Medicinal Plants. 1st Edition, Volume 4. Chennai: Orient Longman Pvt. Ltd.; 2003:33-5.

Rajagopalan PM. Siddha Medicine. Madurai: Siddha Maruthuva Gurukulam; 2000.

Vimala R, Nagarajan S, Alam M, Susan T, Joy S. Antiinflammatory and antipyretic activity of Michelia champaca Linn. (white variety), Ixora brachiata Roxb. and Rhynchosia cana (Willd.) D.C. flower extract. Indian J Exp Biol. 1997;35(12):1310-4.

Dwajani S, Shanbhag TV. Michelia champaca: Wound healing activity in immunosuppressed rats. Internet J Alternative Med. 2009;7(2):6. Available at Cited 07 Sep 2012.

Shanbhag TV, Kodidela S, Shenoy S, Amuthan A, Kurra S. Effect of Michelia champaca Linn flowers on burn wound healing in Wistar rats. Int J Pharm Sci Rev Res. 2011;7(2):112-5.

Bairy KL, Satish KMC, Savin CG, Kiran KN, Avinash M. Effect of different formulations of silver sulphadiazine cream on experimentally induced burn wound healing. Res J Pharm Biol Chem Sci. 2012;3(2):884-9.

Ganesh B, Sanjeeva, Bairy KL. Effect of Tridax procumbens on burn wound healing. Indian Drugs. 2003;40(8):488-91.

Acute Oral Toxicity – Up-and-Down-Procedure (UDP). OECD Guidelines for the Testing of Chemicals. OECD; 2008. Available at Cited 08 Sep 2012.

Low-Dose Streptozotocin Induction Protocol. Animal Models of Diabetic Complications Consortium Protocols, 2003 Apr 9. Available at Cited 08 Sep 2012.

Vogel HG, Muller G, Herling AW. Methods to induce experimental diabetes mellitus. In: Vogel HG, Vogel WH, Scholkens BA, Sandow J, Muller G, Vogel WF, editors. Drug Discovery and Evaluation: Pharmacological Assays. 2nd Edition. New York: Academic Press; 2007.

Perez Gutierrez RM, Vargas SR. Evaluation of the wound healing properties of Acalypha langiana in diabetic rats. Fitoterapia. 2006;77(4):286-9.

Ehrlich HP, Hunt TK. The effects of cortisone and anabolic steroids on the tensile strength of healing wounds. Ann Surg. 1969;170(2):203-6.

Lee KH. Studies on the mechanism of action of salicylate. II. Retardation of wound healing by aspirin. J Pharm Sci. 1968;57(6):1042-3.

Morton JJ, Malone MH. Evaluation of vulneray activity by an open wound procedure in rats. Arch Int Pharmacodyn Ther. 1972;196(1):117-26.

Kumar MS, Kirubanandan S, Sripriya R, Sehgal PK. Triphala promotes healing of infected full-thickness dermal wound. J Surg Res. 2008;144(1):94-101.

Gutierrez RMP, Solis RV. Wound healing properties of triterpenes from Buddleia scordioides in diabetic rats. Pharm Biol. 2008;46(9):647-653.

Ramamurthy NS, Kucine AJ, McClain SA, McNamara TF, Golub LM. Topically applied CMT-2 enhances wound healing in streptozotocin diabetic rat skin. Adv Dent Res. 1998;12(2):144-8.

Fujii A, Kobayashi S, Kuboyama N, Furukawa Y, Kaneko Y, Ishihama S, et al. Augmentation of wound healing by royal jelly (RJ) in streptozotocin-diabetic rats. Jpn J Pharmacol. 1990;53(3):331-7.

Pelegrinelli FF, Thirone AC, Gasparetti AL, Araujo EP, Velloso LA, Saad MJ. Early steps of insulin action in the skin of intact rats. J Invest Dermatol. 2001;117(4):971-6.

Goodson WH 3rd, Hunt TK. Wound collagen accumulation in obese hyperglycemic mice. Diabetes. 1986;35(4):491-5.

Bitar MS. Insulin-like growth factor-1 reverses diabetes-induced wound healing impairment in rats. Horm Metab Res. 1997;29(8):383-6.

Hennessey PJ, Black CT, Andrassy RJ. Epidermal growth factor and insulin act synergistically during diabetic healing. Arch Surg. 1990;125(7):926-9.

Komesu MC, Tanga MB, Buttros KR, Nakao C. Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology. 2004;11(2):63-67.

Spanheimer RG, Umpierrez GE, Stump f V. Decreased collagen production in diabetic rats. Diabetes. 1988;37(4):371-6.

Grotendorst GR, Martin GR, Pencev D, Sodek J, Harvey AK. Stimulation of granulation tissue formation by platelet-derived growth factor in normal and diabetic rats. J Clin Invest. 1985;76(6):2323-9.

Gulcelik MA, Dincer H, Sahin D, Demir OF, Yenidogan E, Alagol H. Glucan improves impaired wound healing in diabetic rats. Wounds. 2010;22(1):12-6.

Khan MR, Kihara M, Omoloso AD. Antimicrobial activity of Michelia champaca. Fitoterapia. 2002;73(7-8):744-8.

Wei LS, Wee W, Siong JYF, Syamsumir DF. Characterization of antimicrobial, antioxidant, anticancer property and chemical composition of Michelia champaca seed and flower extracts. J Pharm Sci. 2011;4(1):19-24.

Ya C, Gaffney S, Lilley TH, Haslam E. Carbohydrate-polyphenol complexation. In: Hemingway RW, Karchesy JJ, editors. Chemistry and Significance of Condensed Tannins. 2nd Edition. New York: Plenum Press; 1989: 134-45.

Scortichini M, Rossi MP. Preliminary in vitro evaluation of the antimicrobial activity of triterpenes and terpenoids towards Erwinia amylovora (Burrill). J Bacteriol. 1991;71:109-12.

Lien YH, Tseng MM, Stern R. Glucose and glucose analogs modulate collagen metabolism. Exp Mol Pathol. 1992;57(3):215-21.

Clark RAF. Wound repair: overview and general considerations. In: Clark RAF, editor. The Molecular and Cellular Biology of Wound Repair. 2nd Edition. New York: Plenum Press; 1996:1-48.

Zabel DD, Feng JJ, Scheuenstuhl H, Hunt TK, Hussain MZ. Lactate stimulation of macrophage-derived angiogenic activity is associated with inhibition of Poly(ADP-ribose) synthesis. Lab Invest. 1996;74(3):644-9.

Rajasekaran NS, Nithya M, Rose C, Chandra TS. The effect of finger millet feeding on the early responses during the process of wound healing in diabetic rats. Biochim Biophys Acta. 2004;1689(3):190-201.

Rosenthal SP. Acceleration of primary wound healing by insulin. Arch Surg. 1968;96(1):53-5.

Jarald EE, Joshi SB, Jain DC. Antidiabetic activity of flower buds of Michelia champaca Linn. Indian J Pharmacol. 2008;40(6):256-60.

Clark RA. Potential roles of fibronectin in cutaneous wound repair. Arch Dermatol. 1988;124(2):201-6.

Elizabeth KM, Lakshmi YAS. Antimicrobial activity of Michelia champaca. Asian J Chem. 2006;18(1):196-200.




How to Cite

Gowda, A., Shanbhag, V., Bangalore, E. R., Shenoy, S., Prabhu, K., Narayanareddy, M., & Shanbhag, T. (2017). The wound healing property of ethanolic extract of Michelia champaca flowers in diabetic rats. International Journal of Basic & Clinical Pharmacology, 3(6), 1036–1042. Retrieved from



Original Research Articles