Published: 2017-01-28

Attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp in Swiss albino mice

Chandrashekar R., Manohar V. R., Poovizhi Bharathi R., Mohandas Rai


Background: The objective of this study was to evaluate the attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp (AETBFP) by using elevated plus maze test and dark and light arena models.

Methods: Thirty Swiss albino mice were divided into five groups, Group I received vehicle (1% Gum acacia suspension, 3ml/kg, orally), Group II received standard drug Diazepam (1mg/kg, orally) and Group III, IV and V received AETBFP 9, 18 and 36 mg/kg, orally respectively. In elevated plus maze test, the mouse was placed on the central platform facing towards open arm. The percentage of time spent and frequency of entries and number of rears in open arm was counted for a period of 5 min. In dark and light arena, the time spent, number of entries and number of rears in light arena was counted for a period of 5 min. The mean±SEM values were calculated for each group. The data was analyzed using one-way ANOVA followed by Dunnet’s multiple comparison tests; p< 0.05 was considered as statistically significant.

Results: Significant (p<0.05) reduction in anxiety was noted in experimental animals when given at a dose of AETBFP (36mg/kg), where number of entries and duration of stay in open arm and light arena increased in elevated plus maze and light and dark arena respectively when compared with control animals.

Conclusions: Our study reveals that AETBFP at a dose of 36mg/kg has significant attenuation of anxiety in Swiss albino mice.


Aqueous extract of Terminalia belerica fruit pulp, Dark and light arena, Elevated plus-maze, Gum acacia, Swiss albino mice

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Goodman LS, Gilman. Pharmacological Basis of Therapeutics: Therapy of Depression and Anxiety Disorders.12thed. New York: Macmillan Publishing Co; 2011:187-210.

Kessler RC, Berglund P, Demler O, Jin R, Merikangas KR, Walters EE. Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the National Comorbidity Survey Replication. Arch Gen Psychiatry. 2005;62(6):593-602.

Griffiths RR, Ator NA, Roache JD, Lamb RJ. Abuse liability of triazolam: Experimental measurements in animals and humans. Psychopharmacol Ser. 1987;3:83-7.

Masoumeh E, Mohammad K, Maryam FA. Coriandrum sativum: Evaluation of its anxiolytic effect in the elevated plus-maze. J Ethnopharmacol. 2005;96:365-70.

Lowry CA, Johnson PL, Hay-Schmidt A, Mikkelsen J, Shekhar A. Modulation of anxiety circuits by serotonergic systems. Stress. 2005;8:233-46.

Chen LG, Huang WT, Lee LT, Wang CC. Ellagitannins from Terminalia calamansanai induced apoptosis in HL-60 cells. Toxicology in Vitro. 2009;23:603-9.

Dhanani T, Shah S, Kumar S. A validated high-performance liquid chromatography method for determination of tannin-related marker constituents gallic acid,corilagin, chebulagic acid, ellagic acid and chebulinic Acid in four Terminalia species from India. J Chromatogr Sci. 2015;53(4):625-32.

Mot. Omari NS, Karthikeyan M, Kannan M, Rajasekar S. Terminalia belerica Roxb-A Phytopharmacological Review. IJRPBS 2012;3(1):97.

Diab KA, Guru SK, Bhushan S, Saxena AK. In Vitro Anticancer Activities of Anogeissus latifolia, Terminalia bellerica, Acacia catechu and Moringa oleiferna Indian Plants. Asian Pac J Cancer Prev 2015;16(15):6423-8.

Pinmai K, Chunlaratthanabhorn S, Ngamkitidechakul C, Soonthornchareon N, Hahnvajanawong C. Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells. World J Gastroenterol. 2008;14(10):1491-7.

Manohar VR, Rai MS, Alva A, Dsouza RA, Kateel R. Acute antidepressant activity o acqueous extract of Terminalia belerica fruit in mice in experimental paradigms. Int. J. Res. Ayurveda Pharm. 2014;5(2):198-200.

Manohar VR, Rai M, Chandrashekar R, Sivam S, Kumar BD, Aravind A. Central and peripheral antinociceptive activity of Terminalia belerica fruit pulp aqueous extract in swiss albino mice. IJPP. 2016;3(1):13-6.

Chandrshekar R, Manohar VR, Rao SN, Acute anxiolytic activity of aqueous extract of Terminalia chebula fruit pulp in rats. Int. J. Res. Ayur. Pharm. 2013;4(1):112-15.

Sudhakar P, Gopalakrishna HN, Swathi B, Shreyasi C, Pai MRSM, Nair V. Antianxiety effect of aqueous extract of fruits of Emblica officinalis on acute and chronic administration in rats. JPR. 2010;3(2):219-23.

Suresha RN, Amoghimath S, Vaibhavi PS, Shruthi SL, Jayanthi MK, Kalabarathi HL. Evaluation of Analgesic Activity of Perindopril in Albino Mice. J Adv Pharm Technol Res. 2014;5(3):129-33.

Nagaraja TS, Mahmood R, Krishna V, Thippeswamy BS, Veerapur VP. Evaluation of anxiolytic effect of Erythrina mysorensis Gamb. in mice. Indian J Pharmacol. 2012;44(4):489-92.

Chandrashekar R, Manohar VR, Rao SN. Acute anxiolytic effect of aqueous extract of fruits of Terminalia chebula (AETC) in mice. Int J Pharm Bio Sci. 2012;3(4):643-77.

Vogel HG. Psychotropic and neurotropic activity. In: Drug Discovery and Evaluation: Pharmacological Assays. 3rd ed. New York: Springer; 2008:430-435.

Bhatacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: Anti-anxiety agents. Indian J Exp Biol. 1997;35:565-75.