Neuroprotective effect of secretin in chronic hypoxia induced neurodegeneration in rats

Gowtham Padmanaban, M. K. Kayalvizhi, Kalyanasundaram Kasiviswanathan, Ruckmani Arunachalam, Vishnu Kumar Urkavalan


Background: Hypoxia is a condition in any stage in the delivery of oxygen to cells which include decreased partial pressures of oxygen, less diffusion of oxygen in the lungs, insufficient hemoglobin, inefficient blood flow to the end tissue, and breathing rhythm. Secretin is an amino acid which plays proper functioning of gastro intestinal system.

Methods: The current study was conducted to evaluvate the effect of exogenously administrated secretin on chronic hypoxic damage of brain in rat model. Experimental design consists of control animals, Control animals + secretin hypoxia exposed animals; hypoxia exposed animals +secretin (20ng/

Results: The results of this study point to a possible role of Secretin as neuroprotectant.

Conclusions: Further research on secretin needs to be conducted in order to confirm the deductions made by this study.


Anti-oxidant, Hypoxia, Secretin, Hemoglobin, Neuroprotectant

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Grac R, Sophie. Oxford Handbook of Respiratory Medicine. Oxford University Press; 2004:880.

Illingworth, Robin, Graham, Colin, Hogg, Kerstin. Oxford Handbook of Emergency Medicine. Oxford University Press; 2001:768.

Longmore J, Longmore, Murray, Wilkinson, Ian, Rajagopalan, et al. Mini Oxford Handbook of Clinical Medicine; 2006:874.

Babarczy E, Szabo G, Telegdy G. Effects of secretin on acute and chronic effects of morphine. Pharmacology, Biochemistry and Behavior. 1995;51:469-72.

Chu JY, Yung WH, Chow BK. Ann NY. Secretin. A pleiotrophic hormone. Acad Sci. 2006;1070:27-50.

John D. Bancroft, Marilyn Gamble. Histology methods, Theory and practice of Histological Techniques, edition; 2002.

Franklin RBJ, Paxinos G. The Mouse Brain in Stereotaxic Coordinates. 1997.

Ohkawa H, Ohishi N, Yagi K, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal Biochem. 1979;95(2):351-8.

Bradford MM. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry. 1976;72:248-54.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur J Biochem. 1974;47(3):469-74.

Sinha AK. Colorimetric Assay of Catalase, Analytical Biochemistry. 1972;47(2):389-94.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Science. 1973;179:588-90.

Moron MS, Defierre JW, Mannervik B. Biochem Biophys Acta. 1979;582:67-8.

Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods in Enzymology. 1979;62:3-11.

Rauchova M. Vokurkovai Hypoxia-Induced Lipid Peroxidation in the Brain During Postnatal Ontogenesis H. Physiol. 2001;61:S89-101.

Brenneman DE. Neuroprotection. A comparative view of vasoactive intestinal peptide and pituitary adenylatecyclase-activating polypeptide. Peptides. 2007;28:1720-6.

Bourgault S, Vaudry D, Botia B, Couvineau A, Laburthe M, Vaudry H et al. Novel stable PACAP analogs with potent activity towards the PAC1 receptor. Peptides. 2008;29:919.

Takahashi M, Hasegawa T, Furukawa F, Okamiya H, Shinoda K, Imaida K, et al. Carcinogenesis. 1991;12:2201-4.

Crew FT, Morita Y, Hirata F, Axelrod J, Siraganian RP. Biochemical and Biophysics Research Communications. 1980;93:42-9.