Nephroprotective effect of ethanolic extract of abutilon indicum root in gentamicin induced acute renal failure

Jacob Jesurun RS, Lavakumar S.


Background: The term acute renal failure (ARF) is at present called acute kidney injury (AKI). AKI is a reversible condition in which there is a sudden decline in renal function, manifested by elevated SCr and BUN which occurs in hours to days to weeks. The present study was to evaluate the nephron protective effect of abutilon indicum root in gentamicin induced acute renal failure in wistar albino rats.

Methods: Experimental evaluation was done in gentamicin induced acute renal failure. 24 rats were divided into 6 in each group. Group 1 was given normal saline (10 ml/kg) per oral, group 2 with gentamicin (100 mg/kg, ip for 5 days), group 3 with gentamicin and extract 150 mg/kg and, group 4, gentamicin and extract 300 mg/kg and group 5, extract 300 mg/kg. The dose of the extract was given orally 60 min prior to gentamicin injection. The duration of the study was for 7 days all the doses are given once a day for 7 days. 24 urine output, serum creatinine (SCr), blood urea nitrogen (BUN), total proteins and albumin were measured at end of the day for all the groups. Kidneys were examined for histopathological changes.

Results: Animals treated with extract showed significant improvement in biochemical parameters and histopathological changes compared to group II animal as treated with gentamicin. The protective effect was highly significant the dose of 300 mg/kg of extract.

Conclusions: The ethanolic extract of abutilon indicum root has nephron protective effect in gentamicin induced acute renal failure. Nephro protective action in this study could be due to the antioxidant and other phytochemical of abutilon indicum root.


Abutilon indicum root, Renal failure, Nephro protective, Gentamicin

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Schrier R, Wang W, Poole B, Mitra A. Acute renal failure: definitions, diagnosis, pathogenesis, and therapy. Journal of Clinical Investigation. 2004;114(4):598-8.

Nissenson AR. Acute renal failure: definition and pathogenesis. Kidney Int Suppl. 1998;66:7-10.

Khwaja A. KDIGO clinical practice guidelines for acute kidney injury. Nephron Clin Pract. 2012;120:179-84.

Ali T, Khan I, Simpson W, Prescott G, Townend J, Smith W, et al. Incidence and outcomes in acute kidney injury: a comprehensive population-based study. J Am Soc Nephrol. 2007;18:1292-8.

Hsu C, McCulloch C, Fan D, Ordonez J, Chertow G, Go A. Community-based incidence of acute renal failure. Kidney Int. 2007;72:208-12.

Fang Y, Ding X, Zhong Y, Zou J, Teng J, Tang Y, et al. Acute kidney injury in a chinese hospitalized population. Blood Purif. 2010;30:120-6.

Lafrance J, Miller D. Acute kidney injury associates with increased long-term mortality. J Am Soc Nephrol. 2010;21:345-52.

Uchino S, Bellomo R, Morimatsu H, Morgera S, Schetz M, Tan I, et al. Continuous renal replacement therapy: a worldwide practice survey. Intensive Care Med. 2007;33:1563-70.

Murugan R, Kellum J. Acute kidney injury: what's the prognosis? Nat Rev Nephrol. 2011;7:209-17.

Bharti DT, Raghunath TM, Manojkumar ZC, Namrata VN. Nephroprotective plants: a review. Int J Pharm Pharm Sci. 2012;1:8-16.

Konda VR, Arunachalam R, Eerike M, Rao R, Radhakrishnan AK, Raghuraman LP, et al. Nephroprotective effect of ethanolic extract of Azima tetracantha root in glycerol induced acute renal failure in wistar albino rats. Journal of Traditional and Complementary Medicine. 2015;(6):1-8.

Goyal N, Singh S, Sharma SK, Analgesic effects of various extracts of the root of Abutilon indicum linn. 2009;(1)43-6.

OECD Guidelines for the testing of chemicals (No 423) acute oral toxicity-acute toxic class method.

Erdem A, Gundogan NU, Usubutun A, Kilinc K, Erdem SR, Kara A, Bozkurt A: the protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol Dial Transplant. 2000;15:1175-82.

Kurien BT, Everds NE, Scofield RH. Experimental animal urine collection: a review. Laboratory Animals. 2004;38:333-61.

Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals. J Pharmacol Pharmacother. 2010;1(2):87-93.

Chromy V, Rozkosna K, Sedlak P. Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems. Clin Chem Lab Med. 2008;46:1127-33.

Kabeya T, Ohkura Y. A new colorimetric method for the determination of urea nitrogen in blood with diacetyl monoxime glucuronolactone glucosaccharodilactone reagent. Chem Pharm Bull. 1972;20(4):837-9.

Doumas BT, Bayse DD, Carter RJ, Peters T, Schaffer R. A candidate reference method for determination of total protein in serum. I Development and validation. Clin Chem. 1981;27(10):1642-50.

Affonso A, Lasky F. Bromocresol purple dye-binding method for the estimation of serum albumin adapted to the SMA 12/60. Clinical Biochemistry. 1985;18(5):285-9.

Beauchamp DE, Pettigrew MA. Influence of hydrocortisone on gentamicin-induced nephrotoxicity in rats. Antimicrobial agents and chemotherapy. 19881;32(7):992-6.

Ali BH. The effect of Nigella sativa oil on gentamicin nephrotoxicity in rats. The American journal of Chinese medicine. 2004;32(01):49-55.

Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R, Britti D, et al. A role for superoxide in gentamicin-mediated nephropathy in rats. European journal of pharmacology. 2002;450(1):67-76.