@article{Khare_Kothari_Misra_2017, title={Incidence and sensitivity pattern of Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa in a tertiary care hospital}, volume={6}, url={https://www.ijbcp.com/index.php/ijbcp/article/view/1099}, DOI={10.18203/2319-2003.ijbcp20170324}, abstractNote={<p><strong>Background: </strong>Antimicrobial resistance is a serious problem worldwide and differs from region to region. This study was planned to determine the incidence and sensitivity pattern of <em>Klebsiella pneumoniae (K. pneumoniae)</em>, <em>Escherichia coli (E. coli) </em>and<em> Pseudomonas aeruginosa (P. aeruginosa) </em>in our region and discuss the general issues related to antimicrobial resistance.</p><p><strong>Methods:</strong> Prospective study was carried out between March to October 2015. Samples of urine, blood, pus, CSF and miscellaneous samples (fluids, swabs, sputum and stool) were collected from indoor and outdoor patients for isolation and antimicrobial susceptibility of <em>K. pneumoniae</em>, <em>E. coli </em>and<em> P. aeruginosa </em>in the Department of Microbiology G.R. Medical College, Gwalior (MP).</p><p><strong>Results:</strong> Out of the 5000 samples analyzed 1684 showed growth.<em> K. pneumoniae </em>(38.50%),<em> E. coli </em>(33.29%) and<em> P. aeruginosa</em> (28.19%) constituited a total of 805 isolates. Both <em>E.coli </em>and<em> K. pneumoniae </em>showed highest sensitivity for doxycycline (75%; 67% resp.) and second highest for levofloxacin (70%; 64% resp.), whereas,<em> P. aeruginosa</em> showed highest 57% sensitivity for amikacin followed by 48% for levofloxacin. β-lactam antibiotics and aminoglycosides showed high mean resistance (<em>K.pneumoniae</em>-83%, <em>E.coli</em>-79%, <em>P. aeruginosa</em>-86.4%) and (<em>K. pneumoniae</em>-75%, <em>E. coli</em>-61%, <em>P. aeruginosa</em>-70%) resp.</p><p><strong>Conclusions:</strong> The data indicates high resistance among the gram-negative bacteria for β-lactam and aminoglycoside antibiotics. Increasing resistance to doxycycline and flouroquinolones for <em>K. pneumoniae </em>and<em> E. coli</em> and multidrug resistance to <em>P. aeruginosa</em> is a cause of concern in this region. Thus, there is a need to stop misuse of antibiotics with immediate effect and to implement a strong antimicrobial stewardship program.</p>}, number={2}, journal={International Journal of Basic & Clinical Pharmacology}, author={Khare, Anubhuti and Kothari, Saroj and Misra, Vaibhav}, year={2017}, month={Jan.}, pages={329–333} }