Pharmacological evaluation of anti-ulcer activity of Cymbopogon flexuosus

Authors

  • Sreedevi Adikay Division of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India
  • T. Santhoshini Division of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India

Keywords:

Anti-ulcer activity, Ranitidine, Cymbopogon flexuosus

Abstract

Background: Peptic ulcer disease is a worldwide problem. Currently, there is no cost-effective treatment to relieve pain, heal the ulcer and prevent ulcer recurrence. Hence, there is a dire need to search and find a suitable treatment from natural sources. The present study was designed to investigate the anti-ulcer activity of ethanol extract of the leaves of Cymbopogon flexuosus.

Methods: The ethanol extract of the leaves of Cymbopogon flexuosus was prepared by hot extraction method. Anti-ulcer activity was evaluated in rats and method employed was pylorus ligation. Animals were divided into four groups of six animals each. The animals of Group I served as normal control (vehicle) which received normal saline (5 ml/kg b.wt., p.o). Group II and III received 200 and 400 mg/kg b.wt. of ethanol extract, respectively. The animals of Group IV served as standard control which received ranitidine (15 mg/kg bd.wt.). At the end of study parameters like ulcer index, free acidity, total acidity, acid volume, and pH were determined.

Results: The ethanol extract showed a significant reduction in the total acidity, free acidity, and acid volume. The efficacy of plant extract at high dose was comparable with the standard drug - ranitidine.

Conclusion: Our study results support the ethnomedical use of leaves of C. flexuosus.

References

Devi RC, Sim SM, Ismail R. Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum. J Smooth Muscle Res. 2011;47(5):143-56.

Borrelli F, Izzo AA. The plant kingdom as a source of anti-ulcer remedies. Phytother Res. 2000;14(8):581-91.

Chandrashekar KS, Prasanna KS. Analgesic and anti-inflammatory activities of the essential oil from Cymbopogon flexuosus. Pharmacogn J. 2010;2(14):23-5.

Silva Cde B, Guterres SS, Weisheimer V, Schapoval EE. Antifungal activity of the lemongrass oil and citral against Candida spp. Braz J Infect Dis. 2008;12(1):63-6.

Adinarayana G, Rahul G, Ravi Kiran S, Syamsundar KV, Rao RBR. Evaluation of antimicrobial potential of field distilled and water soluble essential oils of Cymbopogon flexuosus. J Pharmacogn. 2012;3(2):142-6.

Quintans-Junior LJ, Guimaraes AG, Araujo BES, Oliveira GF, Santana MT, Moreira FV, et al. Carvacrol, (-)-borneol and citral reduce convulsant activity in rodents. Afr J Biotechnol. 2010;9(39):6566-72.

Harborne JB, editors. Phytochemical Methods: a Guide to Modern Techniques of Plantanalysis. 3rd Edition. London: Chapman and Hall; 1998: 302.

OECD Test Guideline 423. OECD Guideline for Testing of Chemicals. Acute Oral Toxicity– Acute Toxic Class, 2001. Available at http://www.oecd.org/document/40/0,3343,en_2649_34377_37051368_1_1_1_1,00.html. Accessed 02 Nov 2014.

Shay H, Komarow SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology. 1945;5:43-61.

Ahamthulla M, Asad M, Satya Prasad V. Antiulcer activity of Allium sativum bulb juice in rats. Saudi Pharm J. 2009;17(1):70-7.

Susan G, Sathimoorthy A, Sathimoorthy SS. Effect of alpha tocopherol on gastric ulcers induced by pylori ligation in rats. Indian J Pharmacol. 1990;31:431-3.

Gregory M, Vithalrao KP, Franklin G, Kalaichelavan V. Anti-ulcer (ulcer-preventive)activity of Ficus arnottiana Miq. (Moraceae) leaf methanolic extract. Am J Pharmacol Toxicol. 2009;4(3):89-93.

Rao ChV, Ojha SK, Radhakrishnan K, Govindarajan R, Rastogi S, Mehrotra S, et al. Antiulcer activity of Utleria salicifolia rhizome extract. J Ethnopharmacol. 2004;91(2 3):243-9.

Parmar NS, Desai JK. A review of the current methodology for the evaluation of gastric and duodenal anti-ulcer agents. Indian J Pharmacol. 1993;25:120-35.

Piper DW, Stiel DD. Pathogenesis of chronic peptic ulcer, current thinking and clinical implications. Med Prog. 1986;2:7-10.

de Andrade SF, Lemos M, Comunello E, Noldin VF, Filho VC, Niero R. Evaluation of the antiulcerogenic activity of Maytenus robusta (Celastraceae) in different experimental ulcer models. J Ethnopharmacol. 2007;113(2):252-7.

Ganser AL, Forte JG. K + -stimulated ATPase in purified microsomes of bullfrog oxyntic cells. Biochim Biophys Acta. 1973;307(1):169-80.

Ahmed MF, Thayyil H, Rasheed AS, Ibrahim M. Anti-ulcer activity of Cassia auriculata leaf extract. Pharmacognosy. 2010;2(16):53-57.

Rad AA, Najafzadeh-Varzi H, Farajzadeh-Sheikh A. Evaluation of anti-ulcer activity of Echinops persicus on experimental gastric ulcer models in rats. Vet Res Forum 2010;1(3):188-91.

Deoda RS, Kumar D, Bhujbal SS. Gastroprotective effect of Rubia cordifolia Linn. on aspirin plus pylorus-ligated ulcer. Evid Based Complement Alternat Med 2011;2011:541624.

Raju D, Ilango K, Chitra V, Ashish K. Evaluation of anti-ulcer activity of methanolic extract of Terminalia chebula fruits in experimental rats. J Pharm Sci Res. 2009;1(3):101 7.

Gregory M, Divya B, Mary RA, Hipolith Viji MM, Kalaichelvan VK, Palanivel V. Anti-ulcer activity of Ficus religiosa leaf ethanolic extract. Asian Pac J Trop Biomed. 2013;3(7):554-56.

Babincová M, Schronerová K, Sourivong P. Antiulcer activity of water extract of Scoparia dulcis. Fitoterapia. 2008;79(7-8):587-8.

Goswami M, Kulshreshtha M, Rao CV, Yadav S, Yadav S. Anti-ulcer potential of Lawsonia inermis l. leaves against gastric ulcers in rats. J Appl Pharm Sci. 2011;1:69-72.

Ogle CW, Cho CH, Tong MC, Koo MW. The influence of verapamil on the gastric effects of stress in rats. Eur J Pharmacol. 1985;112(3):399-404.

Jain SM, Parmar NS, Santani DD. Gastric anti-ulcer activity of calcium channel blockers in rats. Indian J Pharmacol. 1994;26:29-34.

Downloads

Published

2017-01-21

How to Cite

Adikay, S., & Santhoshini, T. (2017). Pharmacological evaluation of anti-ulcer activity of Cymbopogon flexuosus. International Journal of Basic & Clinical Pharmacology, 4(2), 208–212. Retrieved from https://www.ijbcp.com/index.php/ijbcp/article/view/898

Issue

Section

Original Research Articles