To evaluate and compare the hypoglycemic, antioxidant and hypolipidemic effect of lycopene with atorvastatin in hyperlipidemic New Zealand white rabbits

Authors

  • Sarita Mulkalwar Department of Pharmacology, Dr. D. Y. Patil Medical College, Pimpri, Pune, Maharashtra, India
  • Bhalchandra Rane Department of Pharmacology, Dr. D. Y. Patil Medical College, Pimpri, Pune, Maharashtra, India
  • Niranjan Munjal Department of Pharmacology, Dr. D. Y. Patil Medical College, Pimpri, Pune, Maharashtra, India
  • Pravin Golande Department of Pharmacology, Dr. D. Y. Patil Medical College, Pimpri, Pune, Maharashtra, India
  • Lopamudra Behera Department of Pharmacology, Dr. D. Y. Patil Medical College, Pimpri, Pune, Maharashtra, India

Keywords:

Antioxidant, Superoxide dismutase, High fat diet

Abstract

Background: During recent years, the carotenoid lycopene has attracted much attention for its potentially beneficial cardiovascular effects, which is located mainly in tomato peels. The antioxidant properties of tomatoes has been extensively studied for the prevention of cardiovascular disease, but the beneficial effects of pure lycopene supplement is still debatable. Therefore, this study was planned to evaluate and compare the hypoglycemic, hypolipidemic and antioxidant effects of pure lycopene powder and to compare it with that of atorvastatin in hyperlipidemic New Zealand white rabbits.

Methods: Adult male New Zealand white rabbits (1.5-2.5 kg) were divided into three groups of six animals each. Group I - High fat diet (HFD) (5 ml/kg). Group II - HFD (5 ml/kg) + lycopene (10 mg/kg) orally. Group III - HFD (5 ml/kg) + atorvastatin (5 mg/kg) orally. Blood samples were taken from all 18 rabbits for baseline estimation of blood sugar levels (BSL), lipid levels and blood superoxide dismutase levels. Same tests were performed in all groups after 6 weeks.

Results: The lipid lowering activity of atorvastatin 5 mg/kg was better than that of lycopene while the antioxidant activity of lycopene was better than that of atorvastatin. Both the results were statistically significant. There was no significant difference in fasting BSLs in both the groups.

Conclusion: Findings suggest that lycopene may have considerable therapeutic benefit as an antioxidant and hypolipidemic agent, but may not be effective as a hypoglycemic agent.

References

Verghese M, Richardson JE, Boateng J, Shackelford LA, Howard C, Walker LT, et al. Dietary lycopene has a protective effect on cardiovascular disease in New Zealand male rabbits. J Biol Sci. 2008;8(2):268-77.

Riccioni G, Mancini B, Di Ilio E, Bucciarelli T, D’Orazio N. Protective effect of lycopene in cardiovascular disease. Eur Rev Med Pharmacol Sci. 2008;12(3):183-90.

Hu MY, Li YL, Jiang CH, Liu ZQ, Qu SL, Huang YM. Comparison of lycopene and fluvastatin effects on atherosclerosis induced by a high-fat diet in rabbits. Nutrition. 2008;24(10):1030-8.

Kamsiah J, Nafeeza M. Effect of nicardipine on fasting plasma lipids and apolipoproteins in male New Zealand white rabbits. Malays J Med Sci. 1999;6(2):5-11.

Rao AV, Rao LG. Carotenoids and human health. Pharmacol Res. 2007;55(3):207-16.

Mangels AR, Holden JM, Beecher GR, Forman MR, Lanza E. Carotenoid content of fruits and vegetables: an evaluation of analytic data. J Am Diet Assoc. 1993;93(3):284-96.

Britton G. Structure and properties of carotenoids in relation to function. FASEB J. 1995;9:1551-8.

Stahl W, Sies H. Lycopene: a biologically important carotenoid for humans? Arch Biochem Biophys. 1996;336(1):1-9.

Rashid S, Uffelman KD, Barrett PH, Lewis GF. Effect of atorvastatin on high-density lipoprotein apolipoprotein A-I production and clearance in the New Zealand white rabbit. Circulation. 2002;106(23):2955-60.

Shyamala MP, Venukumar MR, Latha MS. Antioxidant potential of Synzygium aromaticum (Gaertn.) Linn (Cloves) in rats fed with high fat diet. Indian J Pharmacol. 2003;35:99 103.

Suzuki M, Kakuta H, Takahashi A, Shimano H, Tada-Iida K, Yokoo T, et al. Effects of atorvastatin on glucose metabolism and insulin resistance in KK/Ay mice. J Atheroscler Thromb. 2005;12(2):77-84.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499-502.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47(3):469-74.

Silaste ML, Alfthan G, Aro A, Kesäniemi YA, Hörkkö S. Tomato juice decreases LDL cholesterol levels and increases LDL resistance to oxidation. Br J Nutr. 2007;98(6):1251-8.

Lorenz M, Fechner M, Kalkowski J, Fröhlich K, Trautmann A, Böhm V, et al. Effects of lycopene on the initial state of atherosclerosis in New Zealand White (NZW) rabbits. PLoS One. 2012;7(1):e30808.

Subhash K, Bose C, Agrawal BK. Effect of short term supplementation of tomatoes on antioxidant enzymes and lipid peroxidation in type-II diabetes. Indian J Clin Biochem. 2007;22(1):95-8.

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Published

2017-01-20

How to Cite

Mulkalwar, S., Rane, B., Munjal, N., Golande, P., & Behera, L. (2017). To evaluate and compare the hypoglycemic, antioxidant and hypolipidemic effect of lycopene with atorvastatin in hyperlipidemic New Zealand white rabbits. International Journal of Basic & Clinical Pharmacology, 4(1), 148–152. Retrieved from https://www.ijbcp.com/index.php/ijbcp/article/view/881

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Original Research Articles