Preclinical thyroid hormone profile studies of an ayurvedic medicine Parthadyarishta after chronic administration to male Sprague-Dawley rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20243022Keywords:
Ayurvedic medicines, Heart tonic, Parthadyarishta, Thyroid hormoneAbstract
Background: Parthadyarishta (PRTm) is an Ayurvedic preparation used as a traditional medicine to treat heart disease. This research was designed to study the thyroid hormone profile after chronic administration of this drug.
Methods: Twenty healthy Sprague-Dawley male rats (90-110 g) were randomly divided into a control group and an experimental group. The experimental group was assigned to receive the drug at a dose of 40 ml/kg of body weight orally. After 28 days of treatment, blood samples were collected to carry out biochemical tests to determine the thyroid hormone safety profile. Serum circulating levels of total thyroxine (tT4), total triiodothyronine (tT3), free thyroxine (fT4), free triiodothyronine (fT3), thyroid stimulating hormone (TSH) were measured by Chemiluminescent Microparticle Immunoassay (CMIA) method.
Results: This study showed statistically significant (p=0.008) [21.93%] decrease in tT3 level and statistically significant (p=0.047) [10.73%] increase in fT3 level in the rats. Also, statistically insignificant decrease in tT4 level and increase in fT4 level were observed. There is a [49.96 %] decrease in TSH level (p=0.371). The results suggested that Parthadyarishta have exerted an opposite effect on both the total and free form of T3 by increasing the free form (fT3) though causing decrease in the total form (tT3).
Conclusions: As Parthadyarishta has significant impact on thyroid hormone levels so it is recommended to use it cautiously for chronic use specially in cardiac patients. Further studies are needed to determine the toxicological profile of Parthadyarishta and its effect on cardiovascular system through interference in thyroid function.
References
Verma S, Thuluvath PJ. Complementary and alternative medicine in hepatology: review of the evidence of efficacy. Clin Gastroenterol Hepatol. 2007;5(4):408-16.
Sikder MM, Zulfiquar TN, Chowdhury IH, Akter K, Asha UH, Haque E, et al. Preclinical lipid profile studies of a classical Ayurvedic preparation, Siddha Makardhwaja (SMD), after chronic administration to male Sprague-Dawley rats. Biol Medic. 2018;10(3):1-4.
Thakur K, Patil P, Gawhankar M. Standardization of ayurvedic formulation “ARJUNARISHTA” in terms of physicochemical, spectroscopy and chromatographic techniques. Int J Pharm Sci Res. 2020;11(12):6237-42.
Mehta M. A Comparative Qualitative and Quantitative Estimation of Catechin by HPTLC in Arjunarishta–an Ayurvedic Formulation. IJRAR. 2020;7(2):112-9.
Priya N, Mathur K, Sharma A, Agrawal R, Agarwal V, Acharya J. Effect of Terminalia Arjuna on total platelet count and lipid profile in patients of coronary artery disease. Advan Human Biol. 2019;9(1):98-101.
Soni N, Singh VK. Efficacy and advancement of Terminalia Arjuna in Indian herbal drug research: A review. Trend Appl Sci Res. 2019;1(4):4.
Gupta A, Nishteswar K, Shukla VJ, Ashok B. Evaluation of analgesic activity of Terminalia arjuna (Roxb.) Wight and Arn bark: A tribal claim. AYU (Int Quart J Res Ayurv). 2014;35(4):458-61.
Shengule SA, Mishra S, Joshi K, Apte K, Patil D, Kale P, et al. Anti-hyperglycemic and anti-hyperlipidaemic effect of Arjunarishta in high-fat fed animals. J Ayurv Integrat Med. 2018;9(1):45-52.
Cota D, Mishra S, Shengule S. Arjunarishta alleviates experimental colitis via suppressing proinflammatory cytokine expression, modulating gut microbiota and enhancing antioxidant effect. Molec Biol Rep. 2020;47:7049-59.
Oehme FW. Veterinary toxicology: a historical perspective. Veterinary Toxicology: Basic and Clinical Principles. Elsevier Inc; 2012:33-34.
Abdou KA, Sharkawy A, editors. Some toxicological studies on ivermectin in goats. Proceeding of the 20 Annual meeting of the Egyptian Society of toxicology Bibliotheca Alexandria, Alexandria University, Alexandria, Egypt; 2004:18-19.
Coles EH. Veterinary clinical pathology. Philadelphia (& London): W. B. Saunders;1967:xi + 455.
Chatterjee T. Handbook of laboratory Mice and Rats. Department of Pharmaceutical Technology, Jadavpur University. 1993;157.
Gad SC. The rat-pathology. Animal Models in Toxicology. 1st ed. 1992:88-96.
Swanson D. Annual bibliography on American labor history 1985: Periodicals, dissertations, and research in progress. Labor history. 2008;27(4):1.
Gross MD, Shapiro B, Freitas JE, Maussier ML. In Vitro Laboratory tests of thyroid function. Thyroid Diseases: Basic Science, Pathology, Clinical and Laboratory Diagnose. 1993:197.
Larsen P, Dockalova J, Sipula D, Wu F. Immunoassay of thyroxine in unextracted human serum. J Clin Endocrinol Metabol. 1973;37(2):177-82.
d Steel RG, Torrie JH. Principles and procedures of statistics: a biometrical approach: McGraw-Hill; 1986.
Hannan J. Analysis of Data Using SPSS. Medical and Pharmaceutical Statistics. 1st ed. Dhaka: Sangbed Publishers; 2007:249-92.
Mahayan BK. Significance of Difference in Mean. Methods in Biostatistics for Medical Andresearch Workers. 1997;6:130-55.
Sterling K, Refetoff S, Selenkow HA. T3 thyrotoxicosis: thyrotoxicosis due to elevated serum triiodothyronine levels. Jama. 1970;213(4):571-5.
Hamburger JI. Evolution of toxicity in solitary nontoxic autonomously functioning thyroid nodules. J Clin Endocrinol Metabol. 1980;50(6):1089-93.
Ladenson PW, Bloch KD, Seidman J. Modulation of atrial natriuretic factor by thyroid hormone: messenger ribonucleic acid and peptide levels in hypothyroid, euthyroid, and hyperthyroid rat atria and ventricles. Endocrinol. 1988;123(1):652-7.
Wahner HW, Gorman CA. Interpretation of serum tri-iodothyronine levels measured by the sterling technic. N Engl J Medi. 1971;284(5):225-30.
Nicoloff J, Lum S, Spencer C, Morris R. Peripheral autoregulation of thyroxine to triiodothyronine conversion in man. Hormone Metabol Res Suppl Series. 1984;14:74-9.
Hasan S, Sikder MM, Ali M, Hossain M, Zulfiquar TN, Akter F, et al. Toxicological studies of the ayurvedic medicine" Naradiya Laksmivilasa Rasa" used in sinusitis. Biol Medi. 2016;8(7):1.
Kaplan MM, Utiger RD. Iodothyronine metabolism in liver and kidney homogenates from hyperthyroid and hypothyroid rats. Endocrinol. 1978;103(1):156-61.
Zulfiquar T, Sikder M, Sana T, Chowdhury I, Saha N, Roy S. Organ body weight ratio toxicity studies of an Ayurvedic medicine Chintamanichaturmukh Ras used in vertigo. Int J Pharm. 2017;7:35-40.
Dwivedi S, Chopra D. Revisiting Terminalia arjuna–an ancient cardiovascular drug. J Trad Complement Medi. 2014;4(4):224-31.
Alissa EM, Ferns GA. Heavy metal poisoning and cardiovascular disease. J Toxicol. 2011;2011(1):870125.
Mahbub M, Sikder M, Sony T, Jahan N, Faruque M, Zaman T, et al. Effect of Pijusaballi Rasa on thyroid hormones in Sprague-Dawley rats. Biol Med (Aligarh). 2020;12(3):467.