Effect of tylophora asthmatica on hepatotoxicity induced by antitubercular drugs in albino rats
Keywords:Tylophora asthmatica, Antitubercular drugs, Hepatotoxicity, ALT, AST, Bilirubin
Background: The burden of metabolism and exposure to dangerous chemicals make liver vulnerable to variety of disorders like acute or chronic inflammation, toxin/ drug related hepatitis or cirrhosis. Tuberculosis is a common problem in India and worldwide. Drug induced liver injury (DILI) is a problem of increasing significance, but has been a long standing concern in treatment of tuberculous (TB) infection. Several studies have revealed that, tylophora asthmatica (Burnf.) Merill. (Family: asclepidaceae) commonly known as antmul possess hepatoprotective property. There is less scientific work regarding the effect of Tylophora asthmatica on hepatotoxicity induced by antitubercular drugs, hence the present study was carried out with the aim of evaluating the effect of tylophora asthmatica on antitubercular drug induced hepatotoxicity in albino rats.
Methods: Total 24 Albino rats weighing about 150-200 grams were divided in four groups, 6 animals in each group. In the study, antitubercular drugs: INH (50 mg/kg) and rifampicin (50 mg/kg), test drug: aqueous extract of tylophora asthmatica doses (200 mg/kg and 300 mg/kg) were used. Drugs were given by oral route for 14 days. Biochemical tests for Sr. ALT, AST, bilirubin (total and direct) and histopathological examination of liver was done.
Results: Tylophora asthmatica treated group had no significant rise in values of Sr.ALT, AST, bilirubin (total and direct) and also there were no significant histopathological changes in this group, indicating hepatoprotective effect of tylophora asthmatica.
Conclusions: It was concluded that tylophora asthmatica has hepatoprotective activity in antitubercular drug induced hepatotoxicity.
Malathi R, Gomez MP. Hepatoprotective effect of methanolic leavesextracts of tylophora asthmatica against paracetamol-induced liver damage in rats. J Pharmacol Toxicol. 2007;2(8):737-42.
Jussi J, Saukkonen, Cohn DL, Jasmer RM, Schenker S, Jereb JA, et al. An official ATS statement: hepatotoxicity of antituberculosis therapy. Am J Respir Crit Care Med. 2006;174:935-52.
Sarich TC, Zhou T, Adams SP, Bain AL, Wall RA,Wright JM. A model of isoniazid-induced hepatotoxicity in rabbits. J Pharmacol Toxicol Methods. 1995;34(2):109-16.
Tayal V, Kalra BS, Agarwal S, Khurana N, Gupta U. Hepatoprotective effect of tocopherol against isoniazid and rifampicin induced hepatotoxicity in albino rabbits. Indian J Exp Biol. 2007;45(12):1031-6.
Jeyakumar R, Rajesh R, Meena B, Rajaprabhu D, Ganesan B, Buddhan S, et al. Antihepatotoxic effect of picrorrhiza kurroa onmitochondrial defense system in antitubercular drugs (isoniazid and rifampicin)-induced hepatitis in rats. J Med Plants Res. 2008;2(1):17-19.
Sodhi CP, Rana SV, Mehta SK, Vaiphei K, Attari S, Mehta S. Study of oxidative-stress in isoniazid-rifampicin induced hepatic injury in young rats. Drug and chemical toxicology. 1997;20(3):255-69.
Vipul G, Patel N. Hepatoprotective activity of alcoholic andaqueous extracts of leaves of tylophora indica in rats. Indian J Pharmacol. 2007;39(1):43-7.
Gupta M, Mukhtar HM, Sayeed A. Phyto-pharmacological and plant tissue culture overview of Tylophora indica (burn f.) Merill. J Pharm Sci and Res. 2010;2(7):401-11.
Mujeeb M, Aeri V, Bagri P, Khan SA. Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill. leaves. Int J Green Pharm. 2009;3:125-7.
Ghosh MN. Fundamentals of experimental pharmacology 4th ed. Hiltonand company Kolkota. 2008;25:184.
Reitman S, Frankel S. In vitro determination of transaminase activity inserum. Am J Clin Pathol. 1957;28:56-60.
Marilyn G. The hematoxylins and eosin. In: John D Bancroft, Marilyn Gamble editors. Theory and practice of Histological Techniques. 6th ed. Churchill Livingstone Elsevier; 2008:121-134.
Raju VP, Raghu H, Chandrashekar, Vijayan P, Dhanraj SA, Rao MC, et al. In vitro and vivo. Hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves. Indian J Pharmacol. 2010;42(2):99-104.
Mahajan BK. Methods in Biostatistics. 6th ed. Jaypee Brothers Significance of difference in mean. 2006;9:130-55.
Jiwon K. An overview of drug-induced liver disease; US Pharm. 2005;30:11.
Saraswathy SD, Suja V, Gurumurthy P, Shyamala Devi CS. Effect of liv.100 against antitubercular drugs (isoniazid, rifampicin andpyrazinamide) induced hepatotoxicity in rats. Research paper Indian J Pharmacol. 1998;30:233-8.
Simon JB. Drug-induced liver disease. In: First Principles of Gastroenterology 4th ed. 2000;507-11.
Kalra BS, Aggarwal S, Khurana N, Gupta U. Effect of cimentidine onhepatotoxicity induced by isoniazid-rifampicin combination in rabbits. Indian J Gastrotenterol. 2007;26(1):18-21.
Pal R, Vaiphei K, Sikander A, Singh K, Rana SV. Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats. World J Gastroenterol. 2006;12(4):636-9.
Kothekar MA, Syed RU, Jaju JB, Mateenuddin MD. Effect of the antioxidants alpha-tocopherol acetate and sodium selenite on hepatotoxicity induced by antitubercular drugs in rat. Indian J Physiol Pharmacol. 2004;48(1):119-22.