Anti-dyslipidemic activity of acacia tortilis seed extract in alloxan-induced diabetic rats


  • Neeraj Kumar Agrawal Department of Pharmacology, Himalayan Institute of Medical Science, SRH University, Dehradun, Uttarakhand, India
  • Suyog Sindhu Department of Pharmacology, MRA Medical College, Ambedkarnagar, Uttar Pradesh, India
  • Nitin Kothari Department of Pharmacology, Pacific Medical College, Udaipur, Rajasthan, India
  • Shruti Chandra Department of Pharmacology, JIIU’s Indian Institute of Medical Sciences and Research, Warudi, Badnapur, Jalna, Maharashtra, India
  • Gini Garima Department of Biochemistry, Shaheed Hasan Khan Mewati Govt Medical College, Nalhar, Mewat, Haryana, India
  • Uma Gupta PhD Life Science, University of Rajasthan, Jaipur, Rajasthan, India



Anti-dyslipidemic activity, Acacia tortilis, Alloxan-induced diabetic rats


Background: The present study was carried out to evaluate the anti-dyslipidemic activities of seed extract of acacia tortilis (ATE) in alloxan inducd diabetic rats.

Methods: The Rats were divided into five groups of six animals each. Groups I and II received normal saline, group III received ATE in dose of 100 mg/kg body weight, group IV received ATE in dose of 200 mg/kg b.w.; and group V received standard drug pioglitazone dose 3 mg/kg b.w. Drugs were administered orally once a day for 30 days. At the end of 0th, 10th, 20th  and 30th day, blood was collected to analyse serum glucose, serum insulin, total cholesterol (TC), serum phospholipid (PL), serum triglyceride (TG), Free fatty acids (FFA) and High density lipoprotein (HDL).

Results: The results has been showed that ATE in above doses significantly increase the serum insulin and HDL level but significantly decreased the elevated level of TC, PL, TG , FFA, LDL and VLDL. It also decreased the atherogenic index and coronary risk index level significantly which was comparable with the pioglitazone.

Conclusions: It is concluded that the seed extract of acacia tortilis at the dose of 100 and 200 mg/kg body weight produced significant anti-dyslipidemic activity in alloxan-induced diabetic rats.


Frier BM, Fisher BM. Diabetes mellitus. In: Haslett C, Chilvers ER, Boon NA, Colledge NR, Hunter JAA,eds,19th ed. Davidson’s Principles and practice of medicine; 2002:644.

Centers for disease control and prevention. National Diabetes Fact Sheet. Atlanta, GA: Centers for disease control and prevention, US department of health and human services; 2011. Available at

Imperatore G, Cadwell BL, Geiss LS, Saaddine JB, Williams DE, Ford ES. Thirty-year trends in cardiovascular risk factor levels among U.S. adults with diabetes. Am J Epidemiol. 2004;160(6):531-9.

Morel DW, Chisolm GM. Antioxidant treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res. 1989;30:1827-34.

Murray RK. In: Tamford: Connecticut, 24th ed. Harper's Biochemistry;1999:868.

Goodman LS, Gilman A, LL Brunton, Lazo JS, Parker KL. In: McGraw-Hill: 11th eds, New York. Goodman and Gilman's the Pharmacological Basis of Therapeutics; 2006:2021.

Mitra SK, Gopumadhavan S, Muralidhar TS, Anturlikar SD, Sujatha MB. Effect of a herbomineral preparation D-400 in streptozotocin-induced diabetic rats. J Ethnopharmacol. 1996;54:41-6.

Mahomed IM, Ojewole JA. Hypoglycemic effect of hypoxis hemerocallidea corm (African potato) aqueous extract in rats. Method Fin Exp Clin Pharmacol. 2003;25:617-23.

Huang THW, Kota BP, Razmovski V, Roufogalis BD. Herbal or natural medicines as modulators of peroxisome proliferator-activated receptors and related nuclear receptors for therapy of metabolic syndrome. Basic Clin Pharmacol. 2005;96:3-14.

Rai MK. A review on some antidiabetic plants of India. Ancient Sci Life. 1995;14:42-54.

Loew D, Kaszkin M. Approaching the problem of bioequivalence of herbal medicinal products. Phytother Res. 2002;16:705-11.

Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree Database:a tree reference and selection guide version 4.0. 2009. Available at

Singh KN, Mettal RK, Barthwal KC. Hypoglycemia activity of acacia catechu, acacia suma, and albizzia odoratisima seed diets in normal albino rats. Indian J Med Res. 1976;64(5):754-7.

Chopra RN, Nayar SL, Chopra IC. Acacia catechu wild. Glossary of Indian Medicinal plants. Council of Scientific and Industrial Research. New Delhi, India;1956:7.

Jayasekhar P, Mohanan PV, Rathinam K. Hepatoprotective activity of ethyl acetate extract of Acacia catechu. Indian J Pharmacol. 1997;29:426-8.

Agrawal NK, Gupta U, Misra P, Singh SP, Verma RC. Antidiabetic effects of acacia tortilis seed extract in normal and alloxan-induced diabetic rats. International J Pharma Sci Res. 2013;4(4):1392-7.

Agrawal NK, Gupta U. Evaluation of hypoglycemic and antihyperglycemic effects of acacia tortilis seed extract in normal and diabetic rats. International J Pharm Tech Res. 2013;5(2):330-6.

CPCSEA (Committee for the purpose of control and supervision on experiments on animals). CPCSEA guidelines for laboratory animal facility. Indian J Pharmacol. 2003;35:257-74.

Antia BS, Okokon JE, Okon PA. Hypoglyacaemic effect of aqueous leaf extract of Persea Americana (Mill) on alloxan induced diabetic rats. Indian J Pharmacol. 2005;37:325-6.

Kulkarni SK. Commonly used drugs, their doses and nature of action in laboratory animals. ln: Vallabh Prakashan. 3rd eds. Delhi. Handbook of Experimental Pharmacology; 2005:190-5.

OECD/OCDE. 425guideline. OECD guidelines for testing of chemicals. Acute oral toxicity-Up-and-down-procedure; 2005:1-26.

Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annal of Clinical Biochemistry. 1969;6:24-7.

Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953;41:486-92.

Zilversmit DB, Davis AK, Memphis B, Tenn N. Estimation of phospholipids in biological fluids. Journal of Laboratory and Clinical Medicine. 1950;35:155-60.

Van Handel E, Zilversmit DB. Micromethod for direct determination of serum triglycerides. Journal of Laboratory and Clinical Medicine. 1957;50(1):152-7.

Mosinger F. Photometric adaptation of Dole’s microdetermination of free fatty acids. J Lipid Res. 1965;6:157-9.

Gidez WM, Webb M. Revision of cholesterol determination. J Biochem. 1950;187:97-106.

Scott MD, Heick HM, Heick NB. An improved double antibody radioimmunoassay for the determination in serum, plasma and incubation media. Canadian J Biochem. 1982;60(10):962-6.

Friedewald WT, Levy R, Frederickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clinical Chemistry. 1972;18:499-502.

Abbott RD, Wilson PW, Kannel WB, Castelli WP. High density lipoprotein-cholesterol, total cholesterol screening and myocardial infarction. The Framingham Study. Arterosclerosis. 1988; 8:207-11.

Alladi S, Shanmugasundaram KR. Induction of hypercholesterolemia by supplementing soy protein with acetate generating amino-acids. Nutrition Report International. 1989;40:893-9.

Stephen ND. Insulin, oral hypoglycemic agents and the pharmacology of endocrine pancrease. In: Goodman LS, Gilman A, Brunton LL, Lazo JS, Parker KL. eds. 11th ed. New York. Goodman and Gilman's the Pharmacological Basis of Therapeutics. McGraw Hill; 2006:1639.

Satyanarayanau, Chakrapaniu. ln: Kolkatta, India: Books and Allied private Ltd. 3rd ed. India. Biochemistry; 2007:538-41.

Murali B, Upadhyaya UM, Goyal RK. Effect of chronic treatment with enicostemma littorale in non-insulin dependent diabetic (NIDDM) rats. J Ethnopharmacol. 2002;81:199-204.

Shirwaika RA, Rajendra NK, Kumar CD, Bodla R. Anti-diabetic activity of aqueous leaf extracts of Annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats. J Ethnopharmacol. 2004;91:171-5.

Pushparaj PN, Low HK, Manikandan J, Tan BK, Tan CH. Anti-diabetic effects of cichorium intybus in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2007;111:430-4.

Sharma HL, Sharma KK. Drug therapy of dislipidemias. ln: India:paras medical publisher. 2nd eds. India. Principal of pharmacology; 2012:324-8.

Balamuruga NK, Indra N, Vanithakumar IG. Effect of rifampicin on certain lipid profiles in the liver of albino rats. Indian J Environ Ecoplant. 2009;16:25-8.

Lemhadr IA, Hajj IL, Michel JB, Eddouk SM. Cholesterol and triglycerides lowering activities of car-away fruits in normal and streptozotocin diabetic rats. J Ethnopharmacol. 2006;106:321-6.

Mayne PD. Lipid profile. In: London, Edward Arnold (A division of Hodder Headline Plc). Clinical chemistry in diagnosis and treatment.1996;224-41.

Singh SK, Kesari AN, Gupta RK, Jaiswal D ,Wata LG. Assessment of antidiabetic potential of cynodon dactylon extract in streptozotocin diabetic rats. J Ethnopharmacol. 2007;114:174-9.




How to Cite

Agrawal, N. K., Sindhu, S., Kothari, N., Chandra, S., Garima, G., & Gupta, U. (2017). Anti-dyslipidemic activity of acacia tortilis seed extract in alloxan-induced diabetic rats. International Journal of Basic & Clinical Pharmacology, 5(4), 1389–1396.



Original Research Articles