Antiulcer activity of aqueous and ethanolic extracts of Ocimum sanctum leaves in albino rats

Sowmya Kaniganti, Das MC, Ramesh Bannaravuri


Background: Peptic ulcer is one of the most common gastrointestinal disorders and a major cause of morbidity. The incidence and prevalence of peptic ulcer has been increasing worldwide. Persisting peptic ulcer leads to complications like gastrointestinal bleeding, gastric perforation and pyloric obstruction. The complications further increase the morbidity and mortality. The objectives of this study were to evaluate the antiulcer activity of aqueous and ethanolic extracts of Ocimum sanctum leaves in albino rats.

Methods: 48 apparently healthy wistar albino rats of either sex weighing about 150-200 grams were divided randomly into 12 groups (n=4). All the drugs were given as pre-treatment, per oral daily for 3 days. Test drugs, aqueous leaf extract (100 mg/kg, 200 mg/kg) and ethanolic leaf extract (50 mg/kg, 100 mg/kg) in 1% carboxymethylcellulose (CMC) were administered. Pyloric ligation and cold restrain stress were used as experimental models in the study. Parameters like free acid, gastric volume and ulcer index were observed.

Results: All the test drugs significantly reduced the free acid, gastric volume and ulcer index in pyloric ligation model. 100 mg/kg of aqueous leaf extract failed to decrease the ulcer index in cold restrain stress, but other doses of test drugs reduced the ulcer index.

Conclusions: Both the doses of aqueous and ethanolic extracts of Ocimum sanctum leaves exhibited significant antiulcer activity in pyloric ligation model, whereas only 200 mg/kg of aqueous leaf extract was effective in cold restrain stress model.


Pyloric ligation, Cold restrains stress, CMC, Ranitidine, Ocimum sanctum leaves extracts

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Alvin CP. Diabetes mellitus. In: Anthony SF, Dennis LK, Dan LL, Eugene B, Stephen LH, Jameson JL, Joseph L editors. Harrison’s principles of internal medicine. 17th ed. United States of America: Mc graw hill companies pvt ltd; 2008:2294.

Shagufta N, Javed Ahmed K, Ghufran A. Some experimentally proved herbs in peptic ulcer disease. Int J Pharm Sci Res. 2012;3(8):2387-92.

Fu We W, Ming ST, Guang YM, Hung YC, Hsien CY, Lung Ch C, et al. Prevalence and risk factors of asymptomatic peptic ulcer disease in Taiwan. World J Gastroenterol. 2011;17(9):1199-203.

Swetha G, Pramod KM, Surender S, Sharma KK, Rimi S. Antidiabetic antihypercholesterolaemic and antioxidant effect of ocimum sanctum linn seed oil. Ind J Exp Bio. 2006;44:300-4.

Ghangale GR, Tushar M, Jadhav ND. Evaluation of antiulcer activity of ocimum sanctum in rats. Veterinary world. 2009;2(12):465-6.

Bharat BM, Das MC, Subas CD, Nagoji KEV. Antiulcer activity of aqueous and ethanolic leaf extracts of Krishna tulasi (ocimum sanctum) in albino rats. Res J Pharm Bio Chem Sci. 2013;4(2):961-6.

Harshal F, Yaseen G, Amit V, Sagar B, Sudhakar J, Nikku Y. Evaluation of ulcer protective effects of ocimum sanctum and zingiber officinalis as herbo formulation for aspirin induced ulcerations in albino rats. Der Pharmacia Sinica. 2011;2(2):46-53.

Ramaswamy S, Sengottuvelu S, Sherief SH, Jaikumar S, Saravanan R, Prasad Kumar C, et al. Gastroprotective activity of ethanolic extract of trachyspermum ammi fruit. Int J Pharm Biosci. 2010;1(1):1-15.

Patchareewan P, Jintanaporn W, Orapin P, Wichittra T, Bunkerd K. Antigastric ulcer effect of turmeric in rats. Srinagarind Med J. 2000;15(4):250-4.

Atta AH, Soad MN, Samar MM. Anti ulcerogenic effect of some plant extracts. Natural product radiance. 2005;4(4):258-63.

Sumalatha G, Bahlul ZA, Baburao C, Mukkanti K. Anti ulcerogenic and ulcer healing studies of aqueous extract of peurparia tuberosa leaves on rats. Int J Pharm Bio Sci. 2010;1(4):657-61.

Manish AR, Sunita MJ. Gastroprotective effect of benincasa hispida fruit extract. Ind J Pharmacol. 2008;40(6):271-5.

Pankaj K, Sunil S, Suman, Suresh K. Antiulcer effect of methanolic extract of tamarindus indica seeds in different experiment models. J Pharm Bioallied Sci. 2011;3(2):236-41.

Jaikumar S, Ramaswamy S, Arokam BR, Mohan T, Gnanavel M. Antiulcer activity of methanolic extract of jatropha curcas linn on aspirin induced gastric lesions in wistar strain rats. Res J Pharm Bio Chem Sci. 2010;1(4):886-97.

Ponnudurai K, Prabhu K, Jebasingh D, Prabu D. Evaluation of antiulcer activity of ethanolic extract of abutilon indicum linn sweet leaves. Der Pharmacia Sinicia. 2011;2(4):148-58.

Yogendr B, Kalpana P, Mohan Singh MR, Sunil J, Sampada U. Antiulcer activity of melia azedarach linn in aspirin induced and pylorus ligated rats. J Pharm Res. 2009;2(9):1456-9.

Anurag K, Uma Shankar S, Rao Ch V. Experimental evaluation of ficus racemosa linn fruits extract on gastric ulceration. Int J Pharm Sci Rev Res. 2010;4(3):89-92.

Vidya S, Ramesh A, Alekhya N, Lohith I. Antiulcer activity of Eugenia jambolana leaves against ethanol induced gastric ulcers in albino rats. Int J Pharm Res Dev. 2011;3(5):106-12.

Muhammed Ashraf VK, Thamotharan G, Sengottuvelu S, Sherief HS, Sivakumar T. Evaluation of antiulcer activity of ficus pumila linn leaf extract in albino rats. Global J Res Med Plants Indigen Med. 2012;1(8):340-51.

Nilesh M, Dinesh S, Dhirendra S. Evaluation of antiulcer potential of leaves of jasminum grandiflorum. Int J Ph Sci. 2009;1(2):247-9.

Mohammed Safwan AK, Syed Ahmed H, Abdul Manam MJ, Zainul AZ, Mohib K. Antiulcer activity of ficus relgiosa stem bark ethanolic extract in rats. J Med Plants Res. 2011;5(3):354-9.

Thamotharan G, Sekar G, Ganesh T, Sen S, Raja C, Senthil Kumar N. Antiulcerogenic effects of lantana camara linn leaves on in vivo test models in rats. Asian J Pharm Clin Res. 2010;3(3):57-60.

Samuel AO, Neetu S, Samuel BO, Vaibhav M, Gautam P. Antiulcer and antioxidant activities of hedranthera barteri with possible involvement of H+ K+ ATPase inhibitiory activity. Ind J Med Res. 2010;132:442-9.

Kenneth R, McQuaid MD. Basic and clinical pharmacology. 11th ed. Tata McGraw Hill Education Private Limited; 2009:1069-71.

Deshmukh VN, Nehete JY, Shewale VV, Raghav NA, Gawande VT. Gastric antiulcer activity of achyranthes aspera linn roots in pylorus ligated rats. Global J Pharmacol. 2011;5(3):143-6.