Published: 2019-11-25

Effect of herbal combination of triphala and Garcinia cambogia extracts on liver function test and kidney function test in high fat diet induced obesity in rats

Vijay Kumar A. N., Vijay Thawani, Lal Hingorani


Background: Obesity, a global epidemic, is a major risk factor for diabetes mellitus and cardio vascular diseases. Despite advances, the pharmacotherapy for obesity remains limited. Almost all medications for long term management of obesity have health issues. Due to the adverse drug reactions (ADRs) associated with many antiobesity medicines, the clinical trials are focussing on screening herbal medicines for use in the treatment of obesity, which have minimal ADRs.

Methods: Rats were divided into eight groups of six each. The rats were first made obese by feeding high fat diet (HFD) for three weeks. Then treatment with the herbal extracts was given simultaneously with the HFD to the experimental groups. Rats were fed HFD for six weeks along with herbal extracts and the effect on their liver function test and kidney function test were evaluated.

Results: The rats fed HFD and supplemented with herbal preparations of Triphala and G. cambogia for six weeks, showed significant improvement in liver function test and kidney function test related parameters as compared to the control group rats fed with HFD alone.

Conclusions: Triphala and G. cambogia can counter the effects of HFD intake and have the potential for use as anti-obesity agents with desirable liver function test and kidney function test related parameters modulating properties.


Obesity, Triphala, Garcinia cambogia

Full Text:



Chavarro JE, Toth TL, Wright DL, Meeker JD, Hauser R. Body mass index in relation to semen quality, sperm DNA integrity, and serum reproductive hormone levels among men attending an infertility clinic. Fertil Steril. 2010;93(7):2222-31.

Mayes JS, Watson GH. Direct effects of sex steroid hormones on adipose tissues and obesity. Obes Rev. 2004;5(4):197-216.

Pasquali R, Pelusi C, Genghini S, Cacciari M, Gambineri A. Obesity and reproductive disorders in women. Hum Reprod Update. 2003;9(4):359-72.

Robker RL. Evidence that obesity alters the quality of oocytes and embryos. Pathophysiology. 2008;5(2):115-21.

Hammoud AO, Wilde N, Gibson M, Parks A, Carrell DT, Meikle W. Male obesity and alteration in sperm parameters. Fertil Steril. 2008;90(6):2222-5.

Archer ZA, Mercer JG. Brain responses to o besogenic diets and diet induced obesity. Proc Nutr Soc. 2007;66(1):124-30.

Tan HM, Gundlach AL, Morris JM. Exaggerated feeding response to central galanin-like peptide administration in diet-induced obese rats. Neuropeptides. 2005;39(3):333-6.

Hill JO, Melanson EL. Overview of the determinants of overweight and obesity, current evidence and research issues. Med Sci Sports Exerc. 1999;31(11):515-21.

Palatty PL, Saldanha E. Pharmacotherapy for weight management. JAPI. 2012;60(3):34-45.

Megraj KVK, Balaraman R, Meenakshisundaram K. Biological activities of some Indian medicinal plants. J Advan Pharm Educ Res. 2011;1(1):12-44.

Knowler W, Pettitt D, Saad M, Bennett P. Diabetes mellitus in the Pima Indians: incidence, risk factors and pathogenesis. Diabetes Metab Rev. 1990;6(1):1-27.

Stunkard A, Foch T, Hrubec Z. A twin study of human obesity. JAMA. 1986;256(1):51-4.

Hebebrand J, Friedel S, Schäuble N, Geller F, Hinney A. Perspectives: molecular genetic research in human obesity. Obes Rev. 2003;4(3):139-46.

Walley A, Asher J, Froguel P. The genetic contribution to non-syndromic human obesity. Nat Rev Genet. 2009;10(7):431-42.

Smith J, Al-Amri M, Dorairaj P, Snideman A. The adipocytes life cycle hypothesis. Clin Sci. 2006;110(1):1-19.

Macia L, Viltart O, Verwaerde C, Delacre M, Delanoye A, Grangette C. Genes involved in obesity: adipocytes, brain and microflora. Genes Nutr. 2006;1(3-4):189-212.

Korner J, Woods S, Woodworth K. Regulation of energy homeostasis and health consequences in obesity. Am J Med. 2009;122(4-1):S12-8.

O'Rahilly S, Farooqi I. Human obesity: a heritable neurobehavioral disorder that is highly sensitive to environmental conditions. Diabetes. 2008;57(11):2905-10.

Boden G. Role of fatty acids in the pathogenesis of insulin resistance and NIDDM. Diabetes. 1997;46(1):3-10.

Furukawa S. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004;114(12):1752-61.

Mahan LK, Escott-Stump S. Krause’s food, nutrition, and diet therapy. 12th ed. Philadelphia: WB Saunders. 2008. Available at: /work/6084784?selectedversion=NBD20212902. Accessed on 29/07/2019.

Chinetti G, Fruchart JC, Staels B. Peroxisome proliferator-activated receptors and inflammation: from basic science to clinical applications. Int J Obes Relat Metab Disord. 2003;27(3):S41-5.

Anindita D, Sikha B, Biswajit D. Pharmacological activities of Baheda (Terminalia bellerica): a review. J Pharmacog Phytochemistr. 2016;5(1):194-7.

Yokozawa T, Kim HY, Kim HJ, Okubo T, Chu DC, Juneja LR. Amla (Emblica Officinalis Gaertn.) prevents dyslipidaemia and oxidative stress in the ageing process. Br J Nutr. 2007;97(6):1187-95.

Kamali SH, Khalaj AR, Shirin HR, Esfehani MM, Kamalinejad M, Larijani B. A systematic review of the antioxidant, anti-diabetic, and anti-obesity effects and safety of triphala herbal formulation. J Med Plants Res. 2013;7(14):831-44.

Juang LJ, Sheu SJ, Lin TC. Determination of hydrolyzable tannins in the fruit of Terminalia chebula Retz. by high-performance liquid chromatography and capillary electrophoresis. J Sep Sci. 2004;27(9):718-24.

Chattopadhyay RR, Bhattacharyya SK. Plant review Terminalia chebula. Pharmacognos Rev. 2007;23:145-50.

Kirby J, Philip N, Howles Y. Hypolipidemic effect of triphala in experimentally induced hypercholesteremic rats. J Lipid Res. 2004;45(1):89-98.

Ferrara L. The Garcinia cambogia in phytotreatment of obesity: activities of the hydroxycitric acid. Europ Scientific J. 2014;10(21):291-301.

Maruthappan VK, Sakthi S. Hypolipedimic activity A Haritaki in atherogenic diet induced hyperlypidemic rats. J Adv Pharm tech Res. 2010;1(2):229-35.

Sergio W. A natural food, the malabar tamarind, may be effective in the treatment of obesity. Med Hypotheses. 1988;27(1):39–40.

Lowenstein JM. Effect of (–)-hydroxycitrate on fatty acid synthesis by rat liver in vivo. J Biol Chem. 1971;246(3):629–32.

Ohia SE. Safety and mechanism of appetite suppression by a novel hydroxycitric acid extract (HCA-SX). Mol Cell Biochem. 2002;238(1-2):89-103.

Dagogo JS. Human leptin regulation and promise in pharmacotherapy. Curr Drug Targets. 2001;2:181–95.

Kizhakkeveettil A, Jayagopal PS, Rose KK. Hypercholesterolemia and Ayurvedic Medicine: A Case Report. Topics Integrat Health Care. 2011;2(2).

Murali YK, Anand P, Tandon V, Singh R, Chandra R, Murthy PS. Long-term effects of Terminalia chebula Retz. on hyperglycemia and associated hyperlipidemia, tissue glycogen content and in vitro release of insulin in streptozotocin induced diabetic rats. Exp Clin Endocrinol Diabetes. 2007;115(10):641-6.

Yokozawa T, Kim HY, Kim HJ, Okubo T, Chu DC, Juneja LR. Amla (Emblica officinalis Gaertn.) prevents dyslipidemia and oxidative stress in the ageing process. Br J Nutr. 2007;97(6):1187-95.