Antidiabetic activity of Plumeria rubra L. in normal and alloxan induced diabetic mice
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20161540Keywords:
Alloxan, Antidiabetic activity, Plumeria rubraAbstract
Background: Diabetes mellitus is a major source of morbidity in developed countries. In spite of the introduction of hypoglycemic agents, diabetes and related complications continue to be a major medical problem. Our present study aims to investigate the antidiabetic activity of aqueous extract of plumeria rubra (PR) in experimental animals.
Methods: PR extract was subjected to antidiabetic study in alloxan induced diabetic model at three-dose levels 100,200 and 400 mg/kg respectively. It was also tested for hypoglycemic activity at same dose levels. Diabetes was induced by alloxan monohydrate (150 mg/kg, i.p.). PR extracts and standard drug glibenclamide (10 mg/kg, p.o.) was administered to animals for 28 days. The blood glucose, serum cholesterol, serum triglyceride, high density lipoprotein, low density lipoprotein, glycosylated hemoglobin. Body weights were assessed in the experimental animals. Histopathological observations during 28 days treatment were also evaluated.
Results: PR extract induced significant reduction (P<0.001) in fasting blood glucose levels in normal and alloxan-induced diabetic mice. Significant differences were observed in serum lipid profiles, glycosylated haemoglobin by extract treated-diabetic animals, when compared with the diabetic control and normal animals. The protective effect of PR was also evident from the histopathological examination on pancreas, liver and kidney. It reduced the injuries induced by alloxan.
Conclusions: PR exhibited significant antihyperglycemic activity in normal and alloxan-induced diabetic mice. The results of the present study provide support to the traditional usage of the plant in diabetes.
References
Davis SN. Insulin, oral hypoglycemic agents and the pharmacology of the endocrine pancreas. Goodman and Gilmans the Pharmacological Basis of Therapeutics. Brunton, L.L. (Ed.). McGraw-Hill. New York; 2006:1613-45.
Matsui T, Tanaka T, Tamura S, Toshima A. Alpha glucosidase inhibitory profile of catechins and theaflavins. J Agric Food Chem. 2007;55:99-105.
Nagappa AN, Thakurdesai PA, Rao NV, Singh J. Antidiabetic activity of terminalia catappa linn fruits. Journal of Ethnopharmacology. 2008;88:45-50.
Murray CJ, Lopez AD. Mortality by cause for eight regions of the world. global burden of disease study. Lancet. 1997;349:1269-76.
Jarald EE, Joshi SB, Jain DC. Antidiabetic activity of flower buds of michelia champaca Linn. 2008;40:256-60.
Jarald EE, Joshi SB, Jain DC. Diabetes and herbal medicines. Iranian Journal of Pharmacology and Therapeutics. 2008;97-106.
Galicia EH, Contreras AA, Santamaria LA, Ramos RR, Miranda AA, Vega LM, et al. Studies on hypoglycemic activity of Mexican medicinal plants. proc. West Pharmacol Soc. 2002;45:118-24.
Medicinal plants in the south pacific. WHO Regional publications. 1998;19:151.
Coskun O, Kanter M, Korkmaz A, Oter S. Quercetin, a flavonoid antioxidant, prevent and protects streptozotocin-induced oxidative stress and-cell damage in rat pancreas. Pharmacological Research. 2005;51:117-123.
Marles RJ, Farnsworth NR, Antidiabetic plants and their active constituents. Phytomedicine. 1995;2:137-189.
Ahmad M, Akhtar MS, Malik T, Gilani AH. Hypoglycemic actionof flavonoids fraction of cuminum nigrum seeds. Phytotherapy Research. 2000;14:03-106.
Pathak D, Pathak K, Singla AK. Flavonoids as medicinal agents recent advances. Fitoterapia. 1991;62:371-89.
Chakravarti BK, Gupta S, Gambhir SS, Gode KD. Pancreatic betacell regeneration. A novel antidiabetic mechanism of Pterocarpusmarsupium Roxb. Indian Journal of Pharmacology. 1980;12:123-27.
Kokate CK, Purohit AP, Gokhale SB. Text book of pharmacognosy. 33rd ed. Pune; Nirali Prakashan; 2001.
OECD 2001. Acute oral toxicity up and down procedure. OECD guidelines for the testing of chemicals 425. Available at http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECDtg425.pdf.
Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Appa Rao AV. Antihyperglycemic activity of Caralluma attenuata. Fitoterpia. 2003;74:274-9.
Sun JE, Ao ZH, Lu JM, Xu Z, Zhang XM, Dou WF. Antihyperglycemic and antilipidperoxidative effects of dry matter of culture broth of innonotus obliquus in submerged culture on normal and alloxan diabetes mice. Journal of Ethnopharmacology. 2008;118:7-13.
Verma PK, Gaikwad NJ. Hypoglycemic activity of cephalandra indica naud stems. Adv Pharmacol Toxicol. 2006;7:57-60.
Vijay S, Patel V, Chitra P, Prasanna PL, Krishnaraju V. Hypoglycemic effect of aqueous extract of parthenium hysterophorus L. in normal and alloxan induced diabetic rats. Indian Journal of Pharmacology. 2008;40:183-5.
