A pharmacological study on the effect of calendula officinalis extract on wound healing in animals


  • Ranjeet Kumar Department of Pharmacology, Raipur Institute of Medical Sciences (RIMS), Raipur, CG, India
  • Sarju R. Zilate Department of Pharmacology, Raipur Institute of Medical Sciences (RIMS), Raipur, CG, India
  • Sushil Varma Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, Wardha, Maharashtra, India
  • Sweety Kumari Department of Pharmacology, Raipur Institute of Medical Sciences (RIMS), Raipur, CG, India




Calendula Officinalis, Excision wound Model, Incision Wound Model, HECO, Wound healing


Background: Calendula officinalis is one of those plants which have medicinal importance due to its flowers (HECO) has pharmacological activity the important pharmacological parameters have been less investigated properly and can be scientifically proved by reverse pharmacology. It was found of interest to evaluate these properties of extract of flowers of Calendula officinalis.

Methods: Study was conducted in Albino Wistar rats. For evaluating wound healing activity Excision wound model and Incision wound mode were used containing 4 groups each. The wound contraction was studied by tracing the raw wound area on graph paper. Scar area and time for complete epithelisation were measured. The percentage of wound contraction was recorded. The differences between experimental groups were compared by ANOVA followed by Students “t” unpaired test.

Results: In the excision wound healing model, 5% and 10% flowers showed a highly significant reduction in wound area as compared with control. In incision wound model, rats treated with 5% and 10% ointment of Calendula officinalis flowers extract showed highly significant increase in tensile strength as compared to control.

Conclusions: It was found that the Calendula extract enhanced the wound healing in both the models as seen by increased synthesis of connective tissue especially collagen. There was a significant increase in the granuloma tissue. In both the models wound contraction and increased tensile strength was found to be statistically significant.


Kumar KLS, Mustapha MH, Rajbhandari A, Ramakrishnan R. Phytochemical and Pharmacological Studies on Achillea Millefolium (L) Leaves. Res J Pharm Biol Chem Sci. 2011;2(1):24-30.

Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol. 2006 Jun 15;106(1):1-28.

Waheed M, Deeba F, Syed R, Ali Y, Zeeyauddin K, Alshatwi A. Unani drug formulation for its analgesic, inflammatory and non-toxic effect. Int J Pharm Pharm Sci. 2011;3(4):66-72.

Craig WJ. Health-promoting properties of common herbs. Am J Clin Nutr. 1999 Sep 1;70(3):491S-499.

Bakó E, Deli J, Tóth G. HPLC study on the carotenoid composition of Calendula products. J Biochem Biophys Methods. 2002 Oct 1;53(1-3):241-50.

Dumenil G, Chemli R, Balansard C, Guiraud H, Lallemand M. [Evaluation of antibacterial properties of marigold flowers (Calendula officinalis L.) and mother homeopathic tinctures of C. officinalis L. and C. arvensis L. (author’s transl)]. Ann Pharm Françaises. 1980 Jan;38(6):493-9.

Kasiram K, Sakharkar PR, Patil AT. Antifungal activity of Calendula officinalis. Indian J Pharm Sci. 2000;6:464.

Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer Science & Business Media; 2008:900.

OECD guidelines for the testing of chemicals. Acute Oral Toxicity: Up - and - Down Procedure) [Internet] 1998 [Updated 1998 Sep 21; cited 2013 Oct 08]. Available at: http:// http://iccvam.niehs.nih.gov/SuppDocs/Fed Docs/OECD/OECDtg425.pdf.

Youth RA, Simmerman SJ, Newell R, King RA. Ketamine anesthesia for rats. Physiol Behav. 1973;10:633-6.

Morton JJ, Malone MH. Evaluation of vulneray activity by open wound procedure in rats. Arch. Int Pharmacodyn Ther. 1972 Mar;196(1):117-26.

Ehrich HP, Hunk TK. Effect of cortisone and anabolic steroids on tensile strength of healing wound. Ann Surg.1969;170:203-6.

Lee KH. Studies on the mechanism action of salicylates. 3. Effect of vitamin A on wound healing retardation action of aspirin. J Pharm Sci. 1968 Jul;57(7):1238-40.

Somayaji SN, Jacob AP, Bairy KL. Effect of tolmetin and its copper complex on wound healing. Indian J Exp Biol. 1995 Mar;33(3):201-4.

Clark RAF. Cutaneous tissue repair. I. Basic biologic consideration. J. Am. Acad. Dermatol. 1985 Nov 1;13(5):701-25.

Lonac M, Parnham MJ, Plauchithiu M, Brune K. Oxaceprol. An atypical inhibitor of inflammation and joint damage. Pharmacol Res. 1996 Jun;33:367-73.

Iba Y, Shibata A, Kato M, Masukawa T. Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice. International Immunopharmacol. 2004 Dec 20;4(14):1873-80.

Schilling JA. Wound healing. Surg Clin North Am. 1976; 56:859-74.

Clark LA, Moon RE. Hyperbaric oxygen in the treatment of life-threatening soft-tissue infections. Respiratory Care Clinics of North America. 1999;5(2):203-19.

Midwood KS, Williams LV, Schwarzbauer JE. Tissue repair and the dynamics of the extracellular matrix. Int J Biochem Cell Biol. 2004;36(6):1031-7.

Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in man. J Mol Med. 1996 Jun 1;74(6):297-312.

Kumar R, Katoch SS, Sharma S. β - Adrenoceptor agonist treatment reverses denervation atrophy with augmentation of collagen proliferation in denervated mice gastrocnemius muscle. Indian J Exp Biol. 2006;44:371-6.

Bent HH. The biochemistry and medical significance of flavonoids. Pharmacol Thera. 2002;96:80-112.

Kuppast IJ, Nayak PV. Wound healing activity of Cordia dichotoma Forst. f. fruits. Natural Product Radiance. 20065;99-102.

Gerber LE and Erdman JW. Effect of dietary retinyl acetate, β-carotene and retinoic acid on wound healing in rats. Journal of Nutrition. 1982 Aug 1;112(8):1555-64.




How to Cite

Kumar, R., Zilate, S. R., Varma, S., & Kumari, S. (2019). A pharmacological study on the effect of calendula officinalis extract on wound healing in animals. International Journal of Basic & Clinical Pharmacology, 8(6), 1209–1214. https://doi.org/10.18203/2319-2003.ijbcp20192103



Original Research Articles