A retrospective analysis of antibiogram on urinary isolates in a tertiary care hospital, Trivandrum, India

Authors

  • Pramodh K. Department of General Medicine, SK Hospital, Edappazhanji, Trivandrum, Kerala, India
  • Thansila L. T. Intern, The Dale View College of Pharmacy and Research Centre, Punalal, Trivandrum, Kerala, India
  • Pushpajan K. S. Intern, The Dale View College of Pharmacy and Research Centre, Punalal, Trivandrum, Kerala, India
  • Lallu Mariam Jacob Department of Pharmacy Practice, The Dale View College of Pharmacy and Research Centre, Punalal, Trivandrum, Kerala, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20192188

Keywords:

Antibiogram, Antibiotics, Bacteriuria, Resistance, Uropathogens

Abstract

Background: Antimicrobial resistance among bacterial strains is an emerging problem. Urinary tract infections are one of the most common bacterial infections in humans both in the hospital and the community settings. Gram-negative bacilli are the common pathogens isolated in urine. These uropathogens have developed resistance to commonly prescribed antimicrobial agents. This severely limits the effective empirical treatment options. Objective of this study was to determine the prevalence and antibiotic susceptibility patterns of bacterial uropathogens isolated from patients attending a tertiary care hospital Trivandrum.

Methods: Urine samples received for culture in the laboratory between 6 month periods was cultured in MacConkey agar and blood agar plates. The growth from urine cultures was processed for identification and antibiotic susceptibility as per standard methods. The details of urinary pathogens grown from urine samples and their Antibiogram profile were collected from the records.

Results: Out of 924 specimens investigated for significant bacteriuria, 226 shows  positive cultures.  E.coli and Klebsiella were the predominant pathogens isolated. Antibiotics like 3rd generation cephalosporins, fluoroquinolones, and cotrimoxazole are the usual empirical treatment options, these organisms have developed resistance towards the latter which might make the empirical therapy less effective.

Conclusions: Gram-negative bacilli were the predominant pathogens isolated and many were resistant to the commonly prescribed antibiotics. Routine surveillance and monitoring studies need to be constantly conducted to update clinicians on the prevalent pathogens for the rational and empirical treatment of bacteriuria. Beta-lactam beta-lactamase inhibitors, aminoglycosides, carbapenem, and nitrofurantoin showed low resistance so they should be considered as empirical treatment options.

References

Chander A, Shrestha CD. Prevalence of extended-spectrum beta-lactamase producing Escherichia coli and Klebsiella pneumoniae urinary isolates in a tertiary care hospital in Kathmandu, Nepal. BMC Res Notes 2013;6:487.

Sarasu VP, Rani SR. Bacteriological profile and antibiogram of urinary tract infections at a tertiary care hospital. Int J Med Microbiol Trop Dis. 2017;3(3):106-12.

Razak SK, Gurushantappa V. Bacteriology of urinary tract infection and antibiotic susceptibility pattern in a tertiary care hospital in South India. Int J Med Sci Public Health. 2012;1(2):109-12.

Das RN, Chandrashekhar TS, Joshi HS, Gurung M, Shrestha N, Shivananda PG. Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in western Nepal. Singapore Med J. 2006 Apr;47(4):281-5.

Shah LJ, Vaghela GM, Mahida H. Urinary tract infection: Bacteriological profile and its antibiotic susceptibility in Western India. Nat J Med Res. 2015;5(1):71-4.

Priyanka R, Jose P. A study on the bacteriological profile of urinary tract infection in adults and their antibiotic sensitivity pattern in a tertiary care hospital in central Kerala, India. Int J Res Med Sci. 2017 Jan 23;5:666-9.

Prakasam AK, Kumar KD, Vijayan M. A cross sectional study on distribution of urinary tract infection and their antibiotic utilisation pattern in Kerala. Int J PharmTech Res. 2012;4(3):1310-6.

Sood S, Gupta R. Antibiotic resistance pattern of community acquired uropathogens at a tertiary care hospital in Jaipur, Rajasthan. Indian J Community Med. 2012;37(1):39-44.

Jha A, Agrawal M, Neral A, Hishikar R, Mahesh B. Escherichia coli as uropathogen: antibiotic susceptibility profiling. Int J Basic Clin Pharmacol. 2018;7(2):433-8.

Fluit AC, Jones ME, Schmitz FJ, Acar J, Gupta R, Verhoef J. Antimicrobial resistance among urinary tract infection (UTI) isolates in Europe: results from the SENTRY Antimicrobial Surveillance Program 1997. Antonie Van Leeuwenhoek. 2000;77(2):147-52.

Akhtar N, Rahman R, Sultana S. Antimicrobial sensitivity pattern of Escherichia coli causing urinary tract infection in Bangladeshi patients. Am J Microbiol Res. 2016;4(4):122-5.

12. Magdaline J. Changing trends in the Antibiotic susceptibility pattern of Acinetobacter species isolated from clinical specimens over a period of five years from a tertiary care hospital. J Med Scie Cli Resea. 2017;05(06):24144-7.

Khalil A. Prevalence and antibiogram of uncomplicated lower urinary tract infections in human population of Gilgit, Northern Areas of Pakistan. Pak J Zool. 2008 Aug 1;40(4):295-301.

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Published

2019-05-23

How to Cite

K., P., T., T. L., S., P. K., & Jacob, L. M. (2019). A retrospective analysis of antibiogram on urinary isolates in a tertiary care hospital, Trivandrum, India. International Journal of Basic & Clinical Pharmacology, 8(6), 1273–1277. https://doi.org/10.18203/2319-2003.ijbcp20192188

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Original Research Articles