Effects of resveratrol on oxidative stress in high fat diet /streptozocin induced diabetic wistar albino rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20190651Keywords:
Diabetes, Oxidative stress, ResveratrolAbstract
Background: Diabetes mellitus is a common chronic disease. One of the pathophysiology is found to be oxidative stress. This study aims to evaluate the effect of resveratrol on oxidative stress in high fat diet (HFD)/streptozotocin induced diabetic rats.
Methods: Wistar albino rats, fed with HFD rendered diabetic with streptozotocin, were divided into 6 groups, namely the diabetic control treated with vehicle (DC), standard control which received metformin (SC), test groups treated with 5,10, and 20 mg/kg b.w. of resveratrol and combination of half dose of metformin and resveratrol (10 mg/kg)(TC). A group of six normal animals served as normal control (NC), another six as HFD control. Fasting blood glucose, lipid profile and serum MDA and SOD were measured one week after induction of diabetes. The animals were then treated orally for 2 weeks after which the same parameters were repeated. The in-vivo results were analysed by one way ANOVA followed by Tukey’s multiple comparison test.
Results: The DC group demonstrated a increase in the fasting blood glucose compared to NC, HFD control while a significant decrease in the fasting blood glucose was observed with SC, Test groups (p<0.05) as compared to the DC group. TC showed a significant improvement in dyslipidemia compared to their baseline values (p<0.05). There was significant change in the serum MDA level and SOD activity.
Conclusions: Resveratrol improves oxidative stress in diabetic rats.
Metrics
References
Yadav NK, Sathian B, Kalai RS. Assessment of Diabetes Mellitus in India and Nepal. Web Med Central Biochem. 2012;3(6),WMC003544.
Jung JY, Lim Y, Moon MS, Kim JY, Kwon O. Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin induced diabetic rats. Nutr Metab (Lond). 2011;8:18.
Buettner R, Parhofer KG, Woenckhaus M, et al. Defining high fat diet rat models: metabolic and molecular effects of different fat types. J Molecular Endocrinol. 2006;36(3):485-501.
Bray GA, Nielsen SJ, Popkin BM. Consumption of high fructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr. 2004;79:537-43.
Shalam Md, Harish MS, Farhana SA. Prevention of dexamethasone and fructose-induced insulin resistance in rats by SH-01D, a herbal prepara¬tion, Indian J Pharmacol. 2006;38(6):419-22.
Bais SK, Shrirao SG, Shende G, Kochar NI, Jiddewar A, Chandewar AV. Evaluation of effects of rutin on oxidative stress in diabetic rat. Int J Pharm Pharm Sci. 2012;4(Suppl 5):140-5.
Genet S, Kale RK, Baquer NZ. Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissue; Effect of vanadate and fenugreek (Trigonella faenum graeoum). Mol Cell Biochem. 2002;236:7-12.
Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocin-induced diabetic rats. Am J Physiol- Endocrinol Metabol. 2006;290:1.
Kaur S, Cheema, Fariborz, Rashid-Kolvear. Streptozotocin induced increase in cholesterol ester transfer protein (CETP) and its reversal by insulin in transgenic mice expressing human CETP Can. J Physiol Pharmacol. 2003;81(10):997-1004
Rivera L, Mor´on R, Zarzuelo A, Galisteo M. Long-term resveratrol administration reduces metabolic disturbances and lowers blood pressure in obese Zucker rats. Biochem Pharmacol. 2009;77(6):1053-63.
Chang KJ. Effect of taurine and beta alanine on morphological changes of pancreas in streptozotocin induced diabetic rats. Adv Exp Med Biol. 2000;483:571-7.
Lu HE, Jian CH, Chen SF, Chen TM. Hypoglycaemic effects of fermented mycelium of Paecilomyces farinosus (G30801) on high-fat fed rats with streptozotocin-induced diabetes, Indian J Med Res. 2010;131:696-701.
Friedewald WT, Levy RI, Frederickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem. 1972;18:499-502.
Dhale LK, Hill EG, Holman RT. The thiobarbituric acid reaction and the auto-oxidation of poly-unsaturated fatty acid methyl ester. Arch. Biochem. Biophysics. 1962;98:253-61.
Kakkar P, Das B, Viswanathan PN. A Modified Spectrophotometric Assay of Superoxide Dismutase. J Biochem Biophy. 1984:21:330.
Rodrigues B, Can MC, Kong J, Goyal RK, McNeil JH. Strain differences in susceptibility to streptozotocin induced diabetes-effect on hypertriglyceridemia and cardiomyopathy. Cardiovasc Res. 1997;534:199.
Szkudelski T. The mechanism of alloxan or streptozotocinaction in B cells of the rat pancreas. Physiol Res. 2001;50(6):537-46.
Pradeepa R, Rema M, Vignesh J, Deepa M, Deepa R, Mohan V. Prevalence and risk factors for diabetic neuropathy in an urban south Indian population: The Chennai Urban Rural Epidemiology Study (CURES-55). Diabet Med. 2008;25:407-12.
Shahi MM, Haidari F, Shiri MR. Comparison of effect of resveratrol and vanadium on diabetes related dyslipidemia and hyperglycemia in streptozotocin induced diabetic rats. Adv Pharma Bulletin. 2011;1(2):81-6.
U.K. Prospective Diabetes Study Group. Eeffect of intensive blood glucose-control with metformin on complications in overweight patients with type2 diabetes (UKPDS). Lancet. 1998;352:854-65.
Kumar A, Kaundal RK, Iyer S, Sharma SS. Effect of resveratrol on nerve functions, oxidative stress and DNA fragmentation in experimental diabetic neuropathy. Life Sci. 2007;80(13):1236-44.
