Evaluation of anti-inflammatory activity of ethanolic extract of Gmelina arborea in experimental acute and sub-acute inflammatory models in wistar rats

Authors

  • Priya Gandigawad Department of Pharmacology, SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India
  • Basavaraj Poojar Department of Pharmacology, Kasturba Medical College Mangalore (Manipal Academy of Higher Education), Karnataka, India
  • Nandan Hodlur Department of Dravyaguna, BVVS Ayurvedic Medical College and Hospital, Bagalkot, Karnataka, India
  • Ravi K. Sori Department of Pharmacology, SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20185170

Keywords:

Aspirin, Carrageenan paw edema, Cotton pellet granuloma, Gmelina arborea, Inflammation

Abstract

Background: Inflammation is a complex reaction to various injurious agents such as infections, trauma, foreign bodies, tissue necrosis, physical and chemical agents, that consists of vascular responses, migration and activation of leukocytes and systemic reactions. Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are currently the most commonly prescribed drugs for treating pain and inflammation. The traditional NSAIDs usually cause various adverse effects on long term use. Gmelina arborea Linn (Gambhari) belongs to family Verbenaceae. The roots, leaves, flowers, fruits and bark are used for treating different ailments as anthelmintic, analgesic, anti-pyretic, antidiabetic, antimicrobial, diuretic and other common disorders.

Methods: The study was carried out by using inflammatory models in wistar rats. The anti-inflammatory activity of G. arborea was compared with standard drug aspirin. The study parameters for acute inflammation was assessment of reduction in inflammation & the percentage inhibition of the paw edema. The parameter for the sub-acute inflammation was percentage inhibition of the dry granuloma weight.

Results: The low and high dose of G. arborea root extract significantly showed the anti-inflammatory activity when compared to control group. The high dose of G. arborea extract showed comparable results in parameters like reducing inflammation, percentage inhibition of paw edema and dry granuloma weight in acute carrageenan paw edema and sub-acute inflammation cotton pellet granuloma models with standard aspirin treated group.

Conclusions: Since G. arborea root extract was having all the qualities required for anti-inflammatory drug. However, no clear inference can be drawn at this stage and hence we consider the work for further extensive research.

References

Gandigawad P, Hogade AP, Patil PA, Malur PR. Effect of rifampicin, isoniazid on acute and subacute inflammation in male Wistar rats: an experimental study. Int J Basic Clin Pharmacol. 2017 Jan 19;4(4):682-5.

Sori RK, Poojar B, Hodlur N, Tilak A, Korde R, Gandigawad P. Evaluation of analgesic activity of sodium valproate and ethanolic extract of Vitex negundo in experimental analgesic models in wistar rats. Int J Basic Clin Pharmacol. 2018;7:2036-4.

Sori RK, Balaji O, Adiga S, Thomas H. Evaluation of the antipeptic ulcer activity of the seed extract of sesame (Sesamum indicum) in stress induced peptic ulcers in rats. Int J Basic Clin Pharmacol. 2018;7:1131-5.

Rafiee L, Hajhashemi V, Javanmard SH. In vitro and in vivo modulation of LPS and carrageenan-induced expression of inflammatory genes by amitriptyline. J Pharmacy Pharmacog Res. 2017;5(3):144-55.

Tentori L, Graziani G, Porcelli SA, Sugita M, Brenner MB, Madaio R, et al. Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes. Antimicrob Agents Chemotherapy. 1998 Mar 1;42(3):550-4.

Turner RA. Screening Methods in Pharmacology. New York and London: Academic Press Inc;1965.

Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenin edema in rats. J Pharmacol Experimental Therapeut. 1969 Mar 1;166(1):96-103.

Crunkhorn P, Meacock SC. Mediators of the inflammation induced in the rat paw by carrageenin. Br J Pharmacol. 1971 Jul;42(3):392-402.

Henriques MG, Silva PM, Martins MA, Flores CA, Cunha FQ, Assreuy-Filho J, et al. Mouse paw edema. A new model for infl ammation? Braz J Med Biol Res. 1987;20:243-9.

Vinegar R, Truax JF, Selph JL, Johnston PR, Venable AL, McKenzie KK. Pathway to carrageenan-induced inflammation in the hind limb of the rat. InFed Proc. 1987;46:118-26.

Posadas I, Bucci M, Roviezzo F, Rossi A, Parente L, Sautebin L, et al. Carrageenan‐induced mouse paw oedema is biphasic, age‐weight dependent and displays differential nitric oxide cyclooxygenase‐2 expression. Br J Pharmacol. 2004 May 1;142(2):331-8.

Bhabani shankar nayak, P. ellaiah, Subas chandra Dinda, Mansi Khadanga, Haragouri Mishra, Sarala Nayak. Anti-inflammatory activity of flavonoid compound isolated from gmelina arborea fruits extract. Int J Applied Pharmaceut Biol Res. 2017;2(1):1-10

Arora C, Tamrakar V. Gmelina arborea: chemical constituents, pharmacological activities and applications. Int J Phytomed. 2017 Dec 28;9(4):528-42.

Lewis JR. Muscarine, oxazole, imidazole, thiazole, and peptide alkaloids, and other miscellaneous alkaloids. Natural Product Rep. 1995;12(2):135-63.

Kulkarni YA, Panjabi R, Patel V, Tawade A, Gokhale A. Effect of Gmelina arborea Roxb in experimentally induced inflammation and nociception. J Ayurveda Integrative Med. 2013 Jul;4(3):152-7.

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Published

2018-12-24

How to Cite

Gandigawad, P., Poojar, B., Hodlur, N., & Sori, R. K. (2018). Evaluation of anti-inflammatory activity of ethanolic extract of Gmelina arborea in experimental acute and sub-acute inflammatory models in wistar rats. International Journal of Basic & Clinical Pharmacology, 8(1), 128–132. https://doi.org/10.18203/2319-2003.ijbcp20185170

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Original Research Articles