In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn.


  • Bindhu R. Kamath Department of Pharmacology, Government Medical College, Ernakulam, Kerala, India
  • Sabeena Kizhedath Department of Pharmacology, Government Medical College, Ernakulam, Kerala, India



Antioxidant activity, Cassia fistula, DPPH, IC50


Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula  was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.

Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.

Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.

Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.


Danish M, Singh P, Mishra G, Srivastava S, Jha KK, Khosa RL. Cassia fistula Linn. (Amulthus)-An important medicinal plant: A review of its traditional uses, phytochemistry and pharmacological properties. J Nat Prod Plant Resour. 2011;1(1):101-18.

Iyengar MA, Pendse GS, Narayana N. Bioassay of Cassia fistula. L.(aragvadha). Planta medica. 1966 Aug;14(03):289-301.

Rizvi MM, El Hassadi IM, Younis SB. Bioefficacies of Cassia fistula: an Indian labrum. African journal of Pharmacy and Pharmacology. 2009 Jun 30;3(6):287-92.

Bahorun T, Neergheen VS, Aruoma OI. Phytochemical constituents of Cassia fistula . African journal of Biotechnology. 2005;4(13).

Lee CK, Lee PH, Kuo YH. The chemical constituents from the aril of Cassia fistula L. Journal of the Chinese Chemical Society. 2001 Dec 1;48(6A):1053-8.

Kannampalli P, Chandrasekaran VRM, Kuppannan G. Protective effect of Cassia fistula Linn. On diethyl nitrosamine induced hepatocellular damage and oxidative stress ethanol pretreated rats. Biol Res. 2010;43:113-25.

Saraswathy A, Devi SN, Ramasamy D. Antioxidant, heavy metals and elemental analysis of Holoptelea integrifolia Planch. Indian journal of pharmaceutical sciences. 2008 Sep;70(5):683.

Gülçin I, Mshvildadze V, Gepdiremen A, Elias R. Screening of antiradical and antioxidant activity of monodesmosides and crude extract from Leontice smirnowii tuber. Phytomedicine. 2006 May 9;13(5):343-51.

Bhalerao SA, Kelkar TS. Traditional medicinal uses, phytochemical profile and pharmacological activities of Cassia fistula Linn. International Research Journal of Biological Sciences. 2012 Sep;1(5):79-84.

Rajagopal PL, Premaletha K, Kiron SS, Sreejith KR. Phytochemical and pharmacological review on Cassia fistula Linn. The golden shower. International Journal of Pharmaceutical, Chemical & Biological Sciences. 2013 Jul 1;3(3).

Subramaniaom LJ, Zakaria Z, Sreenivasan S. Phytochemical screening, DPPH free radical scavenging and xanthine oxidase inhibitory activities of Cassia fsitula seeds extract. Journal of Medicinal Plants Research. 2011;5(10):1941-7.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958 Apr;181(4617):1199.

Mathangi T, Prabhakaran P. DPPH Free Radical scavenging activity of the extracts of Aquatic fern Marsiekla Quadirifola Linn. Intetnational Journal of Current Microbiology and Applied Sciences. 2013;2(10):534-6.

Irshad M, Zafaryab M, Singh M, Rizvi M. Comparative analysis of the antioxidant activity of Cassia fistula extracts. International journal of medicinal chemistry. 2012;2012. Article ID 157125.

Bhalodia NR, Nariya PB, Acharya RN, Shukla VJ. Evaluation of in vitro antioxidant activity of flowers of Cassia fistula Linn. International Journal of Pharm Tech Research. 2011 Jan;3(1):589-99.

Zhou K, Yu L. Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT-Food Science and Technology. 2004 Nov 1;37(7):717-21.

Abid RI, Mahmood RI, Rajesh KP, Kumara Swamy BE. Potential in vitro antioxidant and protective effect of Cassia fistula Linn. fruit extracts against induced oxidative damage in human erythrocytes. Int J Pharm Pharm Sci. 2014;6:497-505.

Tzekiat L, Chiang LK. Total Phenolics, Total Tannins and Antioxidant Activity of Cassia fistula L. extracts of bark, stem, leaf and root under different age classes. Asian Journal of Pharmaceutical Research and Health Care. 2013;5(2).

Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine. 1996 Jan 1;20(7):933-56.

Siddhuraju P, Mohan PS, Becker K. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food chemistry. 2002 Oct 1;79(1):61-7.

Manonmani G, Bhavapriya V, Kalpana S, Govindasamy S, Apparanantham T. Antioxidant activity of Cassia fistula (Linn.) flowers in alloxan induced diabetic rats. Journal of Ethnopharmacology. 2005 Feb 10;97(1):39-42.




How to Cite

Kamath, B. R., & Kizhedath, S. (2018). In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn. International Journal of Basic & Clinical Pharmacology, 7(5), 849–853.



Original Research Articles