In vitro study on antioxidant activity of methanolic leaf extract of Cassia fistula Linn.

Bindhu R. Kamath; Sabeena Kizhedath

doi:10.18203/2319-2003.ijbcp20181424

Authors

Bindhu R. Kamath Department of Pharmacology, Government Medical College, Ernakulam, Kerala, India
Sabeena Kizhedath Department of Pharmacology, Government Medical College, Ernakulam, Kerala, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20181424

Keywords:

Antioxidant activity, Cassia fistula, DPPH, IC50

Abstract

Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.

Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.

Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.

Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.

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