DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20171029

Pharmacological evidence for the anticonvulsant activity of Tylophora indica in experimental animal models

Hafis TK, Shyamjith Manikkoth, Melinda Sequeira, Roopa P. Nayak

Abstract


Background: Epilepsy is a common neurological disorder. The antiepileptic drugs currently used are unable to manage seizures effectively and are associated with numerous adverse drug effects. Hence, there is a necessity of a newer anticonvulsant drug with high therapeutic index profile. The objective of this pre-clinical research was to investigate the role of Tylophora indica on Maximal electric shock [MES] and Pentylene tetrazole [PTZ] provoked convulsions in Wistar albino rats.

Methods: 36 Wistar albino rats were used for this study, after obtaining ethical clearance. The ethanolic extract of the leaves of Tylophora indica [TIEE] (100 mg/kg, p.o) was used to screen the anticonvulsant effect on MES and PTZ provoked convulsions in Wistar albino rats. In MES seizures, inhibition of the tonic hind limb extension and in PTZ seizures, extent of convulsions was noted.

Results: TIEE (100 mg/kg, p.o) significantly (p<0.001) blocked the hind limb extension due to MES. The same dose also significantly (p<0.001) lessened the extent of convulsions induced by PTZ.

Conclusions: The data suggests that the ethanolic extract of Tylophora indica leaves produce its anticonvulsant effect via different mechanisms since it prevented the hind limb extension induced by MES and decreased the duration of convulsions produced by PTZ.


Keywords


Anticonvulsant, Convulsion, Maximal electric shock, Pentylene tetrazole, Tylophora indica, Wistar albino rats

Full Text:

PDF

References


Rasilingam D, Duraisamy S, Subramanian R.Anticonvulsant activity of bioflavonoid gossypin. Bangladesh J Pharmacol. 2009;4:51-4.

Joy AE, Kunhikatta SB, Manikkoth S. Anticonvulsant activity of ethanolic extract of Moringa concanensis leaves in swiss albino mice. Archives of Medicine and Health sciences. 2012;1(1):6-9.

Sheetal V, Singh SP. Current and future status of herbal medicines. Vet World. 2008;1(11):347-50.

Vaidya ADB, Devasagayam TPA. Current status of herbal drugs in India: an over¬view. J Clin Biochem Nutr¬. 2007;41(1):1-11.

Shyamjith M, Joy AE, Thyagaraju BM, Rao SN. Effect of Tylophora indica on haloperidol induced catalepsy in experimental animal models. Drug Invention Today. 2012;4(12):652-54.

Shyamjith M, Chandrashekar R, Rao SN. Antianxiety activity of Tylophora indica in experimental animal models.Int. J Res Ayur Pharm. 2013;4(1):127-29.

Jahas J, Ranjita K, ManikkothS, Rao SN. Protective role of Tylophora indica ethanolic extract on artesunate induced liver toxicity. Int J Appl Biol Pharm. 2014;5(4):206-10.

Dhiman M, Naik V, Kshirsagar R, Desai DC, Manju SL. Antioxidant activity of hydrochloride salt of tylophorinidine and tylophorinine isolated from aerial parts ofTylohoraindica. Int J Res Ayur Pharm. 2012;3(1):121-24.

Manikkoth S, Deepa B, Joy AE, Rao SN. Anti convulsant activity of Phyllanthus amarus in experimental animal models. Int J Appl Biol Pharm. 2011;2(4):144-49.

Goel R, Goel A. Effect of nebivolol on anticonvulsant property of sodium valproate along with neuropharmacological benefits in rodents models of epilepsy and behaviour in mice. Journal of Clinical and Experimental Neuroscience. 2013;1(1):1-9.

Pal A, Nayak S, Sahu PK, Swain T. Piperine protects epilepsy associated depression: a study on role of monoamines. Eur Rev Med Pharmacol Sci. 2011;15:1288-95.

Bozzi Y, Vallone D, Borrelli E. Neuroprotective role of dopamine against hippocampal cell death. J Neurosci. 2000;20(22):8643-49.

Stefulj J, Bordukalo-Niksic T, Hecimovic H, Demarin V, Jernej B. Epilepsy and serotonin (5HT): Variations of 5HT-related genes in temporal lobe epilepsy. Neuroscience Letters. 2010;478:29-31.

Wen-Juan Z, Ying-Hua M, Jian F, Zhen-Tong M, Li-He G. Increase in drug-induced seizure susceptibility of transgenic mice over expressing GABA transporter-1. Acta Pharmacol Sin. 2003;24(10):991-95.

Johnston GAR. GABA-A receptor channel Pharmacology. Curr Pharma Des. 2005;11:1867-85.

Abdul-ghani A. Changes in γ-aminobutyric acid during different stages of picrotoxin- induced seizure, and the effect of pretreatment with γ-acetylenic GABA and phenobarbital. J Biosci. 1989;14(1):63-67.

Lo¨scher W. Pharmacology of glutamate receptor antagonists in the kindling model of epilepsy. Prog. Neurobiol. 1998;54:721-41.

Michael Rogawski A. Molecular targets versus models for new antiepileptic drug discovery. Epilepsy Res. 2006;68(1):22-8.

Farjo IB, McQueen JK. Dopamine agonist and cobalt-induced epilepsy in rats. Br J Pharmac. 1979; 67:353-360. 262Yuri Bozzi and Emiliana Borrelli. The role of dopamine signaling in epileptogenesis. Front Cell Neurosci. 2013;7(157):1-7.

Yan Q, Jobe PC, Dailey JW. Further evidence of anticonvulsant role for 5-hydroxytryptamine in genetically epilepsy prone rats. Br J of Pharmacol. 1995;115:1314-18.

Shyamjith M, Deepa B, Melinda S, Anu EJ, Roysten R. Assessment of brain dopamine levels to evaluate the role of Tylophora indica ethanolic extract on alcohol induced anxiety in Wistar albino rats. J Young Pharm. 2016;8(2):1-10.