Anti diabetic effect of ethanolic extract of leaves of Ocimum sanctum in alloxan induced diabetes in rats
Keywords:Diabetes mellitus, Alloxan induced diabetes, Hypoglycemic drugs, Glibenclamide, Ocimum sanctum
Background: Diabetes mellitus refers to a group of common metabolic disorders that share the phenotype of hyperglycemia resulting from defects of reduced insulin secretion, decreased glucose utilization and increase in glucose production. It is estimated that there are currently 285 million people worldwide and this number is set to increase to 438 million by the year 2030. India has the highest number of patients with known diabetes worldwide, with a prevalence of 11.6%. The aim of the study was to evaluate the anti diabetic activity of ethanolic extract of leaves of plant Ocimum sanctum in alloxan induced diabetes in rats.
Methods: The study was conducted on 4 groups of 6 rats each to evaluate the hypoglycaemic effect of ethanolic extract of Ocimum sanctum. Glibenclamide was used as a standard drug and the results were compared in reference to it. Tween 80 was given for both normal and diabetic control groups. The fasting blood sugar levels were recorded on 1st, 3rd, 5th, 7th, 10th days by glucometer.
Results: The results indicate that the test compound ethanolic extract of Ocimum sanctum has significant and sustained oral hypoglycaemic activity, comparable with the hypoglycaemic effect of glibenclamide, a sulfonylurea.
Conclusion: The hypoglycaemic potential of the test compound is found to be comparable with that of the standard drug glibenclamide.
Chowdhury TA, Hitman GA. Type 2 diabetes in people of South Asian origin: Potential strategies for prevention. Br J Diabetes Vasc Dis. 2007;7:279-82.
Sicree R, Shaw J, Zimmet P. Diabetes and impaired glucose tolerance. Diabetes Atlas, International Diabetes Federation. 3rd ed. Belgium: International Diabetes Federation 2006: pp15-103.
Serrano-Martín X, Payares G, Mendoza-León A. Glibenclamide, a blocker of K+(ATP) channels, shows antileishmanial activity in experimental murine cutaneous leishmaniasis. Antimicrob Agents Chemother. 2006 Dec;50(12):4214-6.
Kirtikar K, Basu B. In: Indian Medicinal Plants, Periodical Expert Book Agency, New Delhi, India; 1993:pp. 3-6.
Hannan JM, Marenah L, Ali L, Rokeya B, Flatt PR, Abdel-Wahab YH. Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic beta-cells. J Endocrinol. 2006 Apr;189(1):127-36.
Oguanobi NI, Chijioke CP, Ghasi S. Anti-diabetic effect of crude leaf extracts of Ocimum gratissimum in neonatal Streptozotocin-induced Type-2 model Diabetic rats. International Journal of Pharmacy & Pharmaceutical Sciences 2012; 4 (Suppl 5):77.
Ghosh MN. Fundamentals of Experimental Pharmacology, 4th edition. Hilton and Company. pp 14.
Mishra P, Mishra S. Study of antibacterial activity of Ocimum sanctum extract against gram positive and gram negative bacteria. Am J Food Technol 2011;6:336-41.
Thakur K. Anti-inflammatory activity of extracted Eugenol from Ocimum sanctum L. leaves. Rasayan Journal of Chemistry 2009;2(2):472-4.
Choudhary GP. Mast cell stabilizing activity of Ocimum sanctum leaves. International Journal of Pharma and Bio Sciences 2010;1(2):1-11.