Hepatoprotective activity of methanolic leaves extract of Rostellularia procumbens by using carbon tetrachloride intoxicated rats

Authors

  • M. Mamatha Department of Pharmacology, Teegala Ram Reddy College of Pharmacy, Hyderabad, Telangana, India
  • V. Manasa Department of Pharmacology, Teegala Ram Reddy College of Pharmacy, Hyderabad, Telangana, India
  • M. Vijusha Department of Pharmacology, Teegala Ram Reddy College of Pharmacy, Hyderabad, Telangana, India
  • R. Suthakaran Department of Pharmacology, Teegala Ram Reddy College of Pharmacy, Hyderabad, Telangana, India

Keywords:

Rostellularia procumbens, Hepatoprotective activity, Serum glutamate oxaloacetate transaminase, Serum glutamate pyruvate transaminase, Alkaline phosphatase, Total bilirubin, Carbon tetrachloride

Abstract

Background: Rostelluria procumbens is a medicinal plant used traditionally in the treatment of asthma, cough and constipation and as an antioxidant etc. it is rich in phytochemical compounds, which are responsible for its biological properties. The present study focused on evaluation of hepatoprotective activity of methanolic extract of R. procumbens leaf in carbon tetrachloride (CCl4) induced hepatotoxocity in rats.

Methods: In this study, 30 wistar rats were used and grouped into 6 each group contain 6 rats. In this study, CCl4 is used as hepatotoxin. Four groups were treated with CCl4 and taken as disease control, standard, and two test groups. One group was taken as control treated with saline. Blood samples were collected and estimated serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase and total bilirubin, which are key markers of liver function. The rats were sacrificed and livers were isolated and histopatological studies carried out.

Results: On oral administration of methanolic leaves extract of R. procumbens to ethanol intoxicated, rats resulted in significant restoration of enzyme levels and also silymarin at a dose of 25 mg/kg. The reversal of increased serum enzymes in ethanol-induced liver damage by the extract may be due to the prevention of leakage of intracellular enzymes by its membrane stabilizing activity.

Conclusion: The results confirm that R. procumbens have hepatoprotective activity againstCCl4 induced hepatotoxicity and significant hepatoprotection seen at 500 mg/kg dose.

References

Yue-Zhong S. Recent natural products based drug development: a pharmaceutical industry perspective. J Nat Prod. 1998;61:1053-71.

Pandey M, Debnath M, Gupta S, Chikara SK. Phytomedicine: an Ancient approach turning into future potential source of therapeutics. J Pharmacogn Phytother. 2011;3(3):27-37.

Ravishankar B, Shukla VJ. Indian systems of medicine: a brief profile. Afr J Tradit Complement Altern Med. 2007;4(3):319-37.

Kaido TL, Veale DJ, Havlik I, Rama DB. Preliminary screening of plants used in South Africa as traditional herbal remedies during pregnancy and labour. J Ethnopharmacol. 1997;55(3):185-91.

Ward FM, Daly MJ. Liver disease. In: Walker R, Edwards C, editors. Clinical Pharmacy and Therapeutics. 3rd Edition. New York: Churchill Livingstone; 2005: 209.

Baskar Rajan G, Chezhiyan N. Strength and wealth of therapeutic medicinal plants in India. In: Khan IA, Khanum A, editors. Role of Biotechnology in Medicinal and Aromatic Plants, Special Volume on Diseases. Volume 6. Hyderabad: Ukaaz Publications; 2002:151-2.

Mossa JS, Tariq M, Mohsin A, Ageel AM, al-Yahya MA, al-Said MS, et al. Pharmacological studies on aerial parts of Calotropis procera. Am J Chin Med. 1991;19(3-4):223 31.

Mascolo N, Sharma R, Jain SC, Capasso F. Ethnopharmacology of Calotropis procera flowers. J Ethnopharmacol. 1988;22(2):211-21.

Balne D, Pallerla P, Vanapatla SR, Bobbala RK. Hepatoprotective effect of whole plant extract fractions of Marsilea minuta linn. Asian J Pharm Clin Res. 2013;6(3):100 7.

Nikhal SB, Dambe PA, Ghongade DB. Hydroalcoholic extraction of Mangiferaindica (aerial parts) by Soxhletion. Int J Pharma Sci. 2010;2(1):30-2.

Kokate CK, Purohit AP, Gokhale SB. Herbal Medicine Introduction. Pharmacognosy. 42nd Edition. Pune: Niraliprakashan; 2008: Appendices 1-6.

De S, Dey YN, Ghosh AK. Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphaphallus paeoniifolius (Aracea). Int J Pharma Bio Res. 2010;1(5):150-7.

Jain S, Dixit VK, Malviya N, Ambawatia V. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Amorphophallus campanulatus Roxb. tubers. Acta Pol Pharm. 2009;66(4):423-8.

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Published

2017-01-29

How to Cite

Mamatha, M., Manasa, V., Vijusha, M., & Suthakaran, R. (2017). Hepatoprotective activity of methanolic leaves extract of Rostellularia procumbens by using carbon tetrachloride intoxicated rats. International Journal of Basic & Clinical Pharmacology, 3(6), 964–969. Retrieved from https://www.ijbcp.com/index.php/ijbcp/article/view/1169

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Original Research Articles