Anti-microbial sensitivity and resistance of organisms in blood-culture samples from prolonged fever cases: evidence from a tertiary care hospital in West Bengal, India

Authors

  • Somanjana Ghosh Department of Microbiology, Midnapore Medical College & Hospital, Midnapore, West Bengal, India
  • Arista Lahiri Department of Community Medicine, Medical College & Hospital, Kolkata, West Bengal, India http://orcid.org/0000-0001-9486-2565
  • Siddhartha Bera Student, College of Medicine & Sagore Dutta Hospital, Kamarhati, West Bengal, India
  • Soumyajyoti Bandyopadhyay Department of Forensic Medicine & Toxicology, College of Medicine & JNM Hospital, Kalyani, West Bengal, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20184323

Keywords:

Antibiotic, Anti-microbial, Blood culture, Culture and sensitivity, Resistance, Sensitivity, Susceptibility

Abstract

Background: Currently there is a rise in resistance to anti-microbials which is a matter of concern in treatment of systemic infections. Blood culture is considered “gold standard” in diagnosis of suspected systemic infection. The susceptibility to antibiotics thereafter determine the future course of treatment. The current study aims to find out the sensitivity and resistance pattern of the blood culture isolates.

Methods: A cross-sectional study was performed on the blood culture samples sent within 24hours of admission of the adult patients reporting fever for 7 days or more with no history of consumption of any antibiotics within last month. Total 134 blood samples were analysed. The proportion of sensitivity and resistance to anti-bacterial agents was calculated among those samples which showed growth in the culture. Background information of the patients in terms of age, sex and religion were also noted.

Results: Mean age of the patients was 39.33 (±12.19) years. Overall 47.76% were female patients and remaining were male. Among the Hindu patients majority were male while among Muslims majority were female. Of the total number of blood cultures examined 46.27% showed growth of bacteria. Staphylococcus aureus was the most frequently found bacteria isolated in cultures, followed by coagulase negative Staphylococcus and Pseudomonas. Of the frequently used antibiotics, higher sensitivity was seen with vancomycin, amikacin, netilmycin, imipenem, gentamicin. High resistance was observed in use of antibiotics like cefixime, amoxicillin-clavulanic acid and azithromycin.

Conclusions: High level of resistance to several commonly used advanced antibiotics warrant judicial and evidence-based use of these drugs.

 

References

World Health Organization, editor. Antimicrobial resistance: global report on surveillance. Geneva, Switzerland: World Health Organization; 2014:232.

Burchardi H, Schneider H. Economic aspects of severe sepsis: a review of intensive care unit costs, cost of illness and cost effectiveness of therapy. Pharmaco Economics. 2004;22(12):793-813.

Tiru B, DiNino EK, Orenstein A, Mailloux PT, Pesaturo A, Gupta A, et al. The Economic and Humanistic Burden of Severe Sepsis. Pharmaco Economics. 2015 Sep;33(9):925-37.

Runcie H. Infection in a Pre-Antibiotic Era. J Infect Dis Prev Med. 2015 Jul 31;3(2):1-5.

Berendsen EM, Levin E, Braakman R, der Riet-van Oeveren D van, Sedee NJ, Paauw A. Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry. Future Microbiol. 2017;12:1135-45.

Lamy B, Dargère S, Arendrup MC, Parienti JJ, Tattevin P. How to Optimize the Use of Blood Cultures for the Diagnosis of Bloodstream Infections? A State-of-the Art. Front Microbiol, 2016. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863885/.

Yamane N. Blood culture: gold standard for definitive diagnosis of bacterial and fungal infections-from the laboratory aspect. Rinsho Byori. 1998 Sep;46(9):887-92.

Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, Clementi M. The Era of Molecular and Other Non-Culture-Based Methods in Diagnosis of Sepsis. Clin Microbiol Rev. 2010 Jan;23(1):235-51.

Epi InfoTM, CDC. 2017. Available at: https://www.cdc.gov/epiinfo/index.html. Accessed December 2017.

IBM SPSS Software, IBM Analytics. Available at: https://www.ibm.com/analytics/spss-statistics-software. Accessed August 2018.

Karam El-Din A-ZA, Mohamed MA, Gad WH, Lotfy GS. Prevalence of microbial pathogens in blood cultures: an etiological and histopathological study. J Taibah Univ Sci. 2010 Jan;3(1):23-32.

Hossain B, Islam MS, Rahman A, Marzan M, Rafiqullah I, Connor NE, et al. Understanding Bacterial Isolates in Blood Culture and Approaches Used to Define Bacteria as Contaminants: A Literature Review. Pediatr Infect Dis J. 2016 May;35(5):S45.

Hall KK, Lyman JA. Updated Review of Blood Culture Contamination. Clin Microbiol Rev. 2006 Oct;19(4):788-802.

Trojan R, Razdan L, Singh N. Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India. Int J Microbiol. 2016.

Downloads

Published

2018-10-23

How to Cite

Ghosh, S., Lahiri, A., Bera, S., & Bandyopadhyay, S. (2018). Anti-microbial sensitivity and resistance of organisms in blood-culture samples from prolonged fever cases: evidence from a tertiary care hospital in West Bengal, India. International Journal of Basic & Clinical Pharmacology, 7(11), 2173–2177. https://doi.org/10.18203/2319-2003.ijbcp20184323

Issue

Section

Original Research Articles