DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20182092

Evaluation of the effect of piperine per se and its interaction with ondansetron on haloperidol induced catalepsy in Albino mice

Pooja Solanki Mishra, Sapna More, Ashutosh Tiwari, Savita Vyas

Abstract


Background: This study aims to evaluate the per se effect of piperine and its interaction with ondansetron on haloperidol induced catalepsy in swiss albino mice.

Methods: The piperine crystals were separated from crude extract of Piper nigrum. Catalepsy was induced by haloperidol (1mg/kg, i.p.). Control group received 2% gum acacia (10ml/kg), standard group ondansetron (0.5mg/kg), test group piperine (10mg/kg) and combination group ondansetron plus piperine (0.5mg/kg + 10mg/kg), per oral, respectively. In acute study, drugs were administered only once, one hour prior to the haloperidol administration. Whereas in chronic study, catalepsy was determined on the seventh day of treatment.

Results: In acute study, from 60 min onwards after haloperidol administration, ondansetron and ondansetron plus piperine group resulted in significantly lower cataleptic scores than the control treated group. On the other hand, 120 min onwards ondansetron group showed significantly lower cataleptic scores (24.62) as compared to the ondansetron plus piperine group (31.50). In the chronic study, from 60 min onwards, ondansetron and the ondansetron plus piperine resulted in significantly lower cataleptic scores than the control treated group. Also the combination of ondansetron plus piperine was more significantly protective compared to ondansetron alone (P <0.05).

Conclusions: Piperine has the potential to be used as a bioenhancer when combined with other drugs which would reduce the dose of drugs and thereby adverse effects. It may act probably by enhancing the bioavailability as well as by inhibiting the metabolic pathways of other drugs.


Keywords


Bioenhancer, Catalepsy, Ondansetron, Piperine

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References


Casey DE. Tardive dyskinesia: Pathophysiology and animal models. J Clin Psychiat. 2000;61:5-9.

Kulkarni SK, Naidu PS. Tardive dyskinesia: an update. Drugs Today. 2001 Feb 1;37(2):97-119.

Somani RS, Kasture VS, Kasture SB. Haloperidol inhibits (-) bicucullin induced seizures and bicucullin potentiates haloperidol induced catalepsy in mice. Indian J Pharmacol. 1999;31:434-6.

Silva SR, Futuro-Neto HA, Pires JG. Effects of 5-HT3 receptor antagonists on neuroleptic induced catalepsy in mice. Neuropharmacology. 1995 Jan 1;34(1):97-9.

Cochran, Tim. Piperine Cochran Foundation of Medical Research. Revised January 30, 1998. Available at: http://www.cochranfoundation.com /reports/piperin.htm. (4-1-00)

Johri RK, Zutshi U. An Ayurvedic formulation Trikatu and its constituents. J Ethnopharmacol. 1992;37:85-91.

Badmaev V, Majeed M, Norkus EP. Piperine, an alkaloid derived from black pepper increases serum response of beta-carotene during 14-days of oral beta-carotene supplementation. Nutr Res. 1999;19:381-8.

Park IK, Lee SG, Shin SC, Park JD, Ahn YJ. Larvicidal activity of isobutylamides identified in Piper nigrum fruits against three mosquito species. J Agric Food Chem. 2002;50(7):1866-70.

Kokate CK, Textbook of practical pharmacognosy, 4th edn., p 143, Vallabh Prakashan, New Delhi 2003.

Harry Lawless. Oral chemical irritation: psychophysical properties. Chem Senses. 1984;9:143- 55.

Merck Index. An encyclopedia of chemicals, drugs and biologicals. 12th Edn., Merck and Co., Whitehouse Station, NJ; 1996:1286.

Pemminati S, Nair V, Dorababu P, Gopalakrishna HN, Pai MRSM. Effect of ethanolic leaf extract of Ocimum Sanctum on haloperidol induced catalepsy in albino mice. Indian J Pharmacol. 2007;39(2):87-9.

Ferre S, Guix T, Prat G, Jane F, Casas M. Is experimental catalepsy properly measured? Pharmac Biochem Behav. 1990;35:753-7.

Dandiya PC, Bhargava, LP. Arch. Int. Pharmacodyn. Therap. 1968;176:157-67.

Chen J, Raymond K. The role of CYP3A4 and p-glycoprotein in food-drug and herb-drug interactions. Pharmacist. 2006;25(9):732-8.

Lee KW, Everts H, Beynen AC. Essential oils in broiler nutrition. Int J Poult Sci. 2004;3:738-52.

Annamalai AR, Madhavan R. Effects of trikatu and its individual components and piperine on the gastrointestinal tract. Trikatu: a bioavailability enhancer. Indian Drugs. 1989;27:595-604.

Johri RK, Thusu N, Khajuria A, Zutshi U. Piperine-mediated changes in the permeability of intestinal epithelial cells. Biochemical Pharmacol. 1992;43:1401-7.

Fujiwara Y, Naithou K, Miyazaki T, Hashimoto K, Mori K, Yamamoto Y. Two new alkaloids, pipercyclobutanamides A and B, from Piper nigrum. Tetrahedron Letters. 2001 Mar 26;42(13):2497-9.