Pharmacokinetics, tissue residues and efficacy of D-Tylo50/25® (tylosin-doxycycline combination) in broiler chickens

Authors

  • Mohamed Aboubakr Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Egypt
  • Mohamed Elbadawy Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Egypt

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20170334

Keywords:

Chickens, Doxycycline, Efficacy, Pharmacokinetics, Residues, Tylosin

Abstract

Background: Pharmacokinetic study of a commercial tylosin-doxycycline combination product (D-Tylo50/25®) was conducted in broiler chickens following intravenous (IV) and oral (PO) administration at doses of 50 mg/kgb. wt. (tylosin) and 25 mg/kg b. wt. (doxycycline).

Methods: Serum drug concentrations were determined by a validated high performance liquid chromatography (HPLC) using UV detection.

Results: A rapid and nearly complete absorption of both drugs with a mean PO bioavailability of 89.16% (tylosin) and 94.30% (doxycycline), prolonged elimination half-lives, and high tissue penetration with steady state volume of distribution of 6.73L/kg (tylosin) and 5.51L/kg (doxycycline) were observed. Tissue residues were studied following oral administration of each drug alone for fiveconsecutive days and blood and tissue samples were obtained for 10 days after the last dose. Residues of tylosin and doxycyclines showed that kidney, liver and lung contained highest drug residues and completely disappeared from those tissues at 5 and 6 days after the last oral dose, respectively. The efficacies of D-Tylo50/25® and other antibiotics (tiamulin and oxytetracyline) were investigated in broiler chicks experimentally infected by Mycoplasma gallisepticum.

Conclusions: The pharmacokinetics of both drugs was characterized by a rapid and complete absorption, extensive tissue distribution and slow elimination. D-Tylo50/25® is more effective than tiamulin and oxytetracycline against Mycoplasma gallisepticum infection in broilers.

References

Giguere S. Lincosamindes, macrolides, and pleuromutilins. In: Antimicrobial Therapy in Veterinary Medicine. 4thed, Wiley- Blackwell, Ames, IA; 2006:179-190.

Kowalski C, Roliński Z, Zań R, Wawron W. Pharmacokinetics of tylosin in broiler chickens. Polish Journal of Veterinary Science. 2002;5:127-30.

Vannuffel P, Cocito, C. Mechanism of action of streptogramins and macrolides. Drugs. 1996;51(1):20-30.

Atef M, Youssef SA, Atta AH, El-Maaz AA. Disposition of tylosin in goats. Dtsch Tierarztl Wochenschr. 1991;98:451-3.

Goren E, De-Jong WA, Doornenbal P, Laurense T. Therapeutic efficacy of doxycycline hyclate in experimental Escherichia coli infection in broilers. The Veterinary Quarterly. 1998;10:48-52.

Aronson AL. Pharmacotherapeutics of the newer tetracyclines. Journal of the American Veterinary Medical Association. 1980;176:1061-8.

Eliopoulos GM, Moellering RC. Antimicrobial combinations. In: Antibiotics in Laboratory Medicine, 3rd ed. (Lorian, V. ed.), Williams and Wilkins, Baltimore; 1991:432-492.

David H. Mycoplasma gallisepticum infection. In: Shif, Y.M.H.J.; J.R. Bames; A.M. Glissom; L.R. Fadly; McDougald and D.E. Swayne (eds.). Diseases of Poultry, 11th ed. Iowa State Press, Iowa; 2003:722-743.

Stipkovits L, Laber G, Burch DGS. Comparative studies on efficacy of MG bacterin and tiamulin treatment of breeder layers. Proceedings Xth World Veterinary Poultry Association Congress, Sydney, Abst 1993;40:155.

Taha AA, Elsheikh HA, Khalafalla AE, Osman IAM, Abdullah AS. Disposition kinetics of tylosin administered intravenously and intramuscularly in desert sheep and Nubian goats. Veterinary Journal. 1999;158:210-5.

Prats C, El Korchi G, Francesch R, Arboix M, Pérez B. Disposition kinetics of tylosin administered intravenously and intramuscularly to pigs. Research in Veterinary Science. 2002;73:141-4.

Gingerich D, Baggot J, Kowalski J. Erythromycin Antimicrobial Activity and Pharmacokinetics in Cows. Canadian Veterinary Journal. 1977;18:96-100.

Abu-Basha EA, Al-Shunnaq AF, Gehring R. Comparative Pharmacokinetics and Bioavailability of TwoTylosin Formulations in Chickens after Oral Administration. Journal of the Hellenic Veterinary Medical Society. 2012;63:159-65.

Ziv G, Sulman FG. Passage of Polymyxins from Serum into Milk in Ewes. American Journal of Veterinary Research. 1973;34:317-22.

Roudaut B, Moretain JP. Residues of macrolide antibiotics in eggs following medication of laying hens. British Poultry Science. 1990;31:661-75.

Brennan J, Moore G, Poe SE, Zimmermann A, Vessie G, Barnum DA, Wilson J. efficacy of in-feed tylosin phosphate for the treatment of necrotic enteritis in broiler chickens. Poult Science. 2001;80:1451-4.

Abd El-Aty AM, Goudaha A, El Zhourba HH. Pharmacokinetics of doxycycline after administration as a single intravenous bolus and intramuscular doses to non-lactating Egyptian goats. Pharmacological Research. 2004;49:487-91.

Anadon A, Larranaga M, Diaz M, Bringas P, Fernandez M, Cruz M, et al. Pharmacokinetics of doxycycline in broiler chickens. Avian Pathology. 1994;23:79-90.

Laczay P, Semjén G, Lehel J, Nagy G. Pharmacokinetics and bioavailability of doxycycline in fasted and nonfasted broiler chickens. Acta Veterinaria Hungarica. 2001;49(1):31-7.

Ismail, MM, El-Kattan YA. Disposition kinetics of doxycycline in chickens naturally infected with Mycoplasma gallisepticum. British Poultry Science. 2004;45:550-56.

Jha VK, Jayachandran C, Singh MK, Singh SD. Pharmacokinetic data on doxycycline and its distribution in different biological fluids in female goats. Veterinary Research Communications. 1989;13:11-6.

Pashov D, Kanelov I. Influence of age on pharmacokinetics of doxycycline and of formulation containing tylosin and bromhexine in chickens. Proceedings of the 6th EAVPT Congress, Edinburgh; 1994:64-65.

Hantash TM, Abu-Basha EA, Roussan DA, Abudabos AM. Pharmacokinetics and bioequivalence of doxycycline (Providox® and Doxyvet 0-50 S®) oral powder formulations in chickens. International Journal of Poultry Science. 2008;7:161-4.

El-Gendi AYI, Atef A, Aziza MM, Kamel GM. Pharmacokinetic and tissue distribution of doxycycline in broiler chickens pretreated with either:diclazuril or halofuginone. Food and Chemical Toxicology. 2010;48:3209-14.

Elkholy HM, Elkomy AA, Elmajdoub AA, Awidat SK. Comparative Bio-equivalence Study of Dolistin® and Colidox® in Chickens. World Applied Sciences Journal. 2009;6(10):1423-8.

Arzey GG, Arzey KE. Successful treatment of mycoplasmosis in layer chickens with singles those therapies. Australian Veterinary Journal. 1992;19:126-8.

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Published

2017-01-28

How to Cite

Aboubakr, M., & Elbadawy, M. (2017). Pharmacokinetics, tissue residues and efficacy of D-Tylo50/25® (tylosin-doxycycline combination) in broiler chickens. International Journal of Basic & Clinical Pharmacology, 6(2), 383–388. https://doi.org/10.18203/2319-2003.ijbcp20170334

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Original Research Articles