Hepatoprotective and antioxidant activities of Hibiscus sabdariffa petal extracts in Wistar rats

Authors

  • Abba P. Obouayeba Department of Biosciences, Laboratory of Biochemical Pharmacodynamic, Félix Houphouët Boigny University of Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire
  • Lydie Boyvin Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire
  • Gervais M. M’Boh Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire
  • Sekou Diabaté Unity of Physio/Phytopathology, Central Laboratory of Biotechnology, National Agricultural Research Center, 01 BP 1740 Abidjan 01, Côte d’Ivoire
  • Tanoh H. Kouakou Department of Natural Sciences, Laboratory of Biology and Crop Improvement, Nangui Abrogoua University of Abidjan, 02 BP 801 Abidjan 01, Côte d’Ivoire
  • Allico J. Djaman Department of Biosciences, Laboratory of Biochemical Pharmacodynamic, Félix Houphouët Boigny University of Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire, Department of Clinical and Fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire
  • Jean D. N’Guessan Department of Biosciences, Laboratory of Biochemical Pharmacodynamic, Félix Houphouët Boigny University of Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire

Keywords:

Antioxidant activity, Aqueous extract, Hepatoprotective activity, Hibiscus sabdariffa, Petals, Wistar rats

Abstract

Background: Hibiscus sabdariffa is a medicinal plant rich in phytochemical compounds, which is the source of its biological properties. This study on the aqueous extract of H. sabdariffa (AEHS) was conducted to assess its hepatoprotective and antioxidant properties.

Methods: It was carried out with 25 Wistar rats divided into five groups. Two groups were treated with a solution of NaCl 0.9%. One group was treated with silymarin at a dose of 25 mg/kg body weight (BW). Two other groups were treated with the AEHS at different doses (100 and 200 mg/kg BW). The treatments were carried out via oral route and at single dose for 7 days. After injection of 2,4-dinitrophenylhydrazine (DNPH), blood samples were collected for the carrying out of biochemical analyses of oxidative stress markers (thiobarbituric acid reactive substances, ferric reduction antioxidant parameter, and 2,2-diphenyl-1-picrylhydrazyl) and hepatotoxicity (albumin, total and direct bilirubin, alanine transaminase, aspartate aminotransferase, and lactate dehydrogenase).

Results: Three major results were obtained. The hepatotoxicity of DNPH expressed by the rats of Group 1 was significantly different (p<0.05) from those of the other groups (control, 2-4) for both hepatotoxicity and oxidative stress markers. The hepatoprotective and antioxidant properties of the AEHS and confirmation of those of silymarin through the rats of Groups 2-4 were statistically identical (p<0.05) to the control group for markers of hepatotoxicity and oxidative stress.

Conclusion: These results confirm and reinforce certain therapeutic virtues of H. sabdariffa.

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Published

2017-01-27

How to Cite

Obouayeba, A. P., Boyvin, L., M’Boh, G. M., Diabaté, S., Kouakou, T. H., Djaman, A. J., & N’Guessan, J. D. (2017). Hepatoprotective and antioxidant activities of Hibiscus sabdariffa petal extracts in Wistar rats. International Journal of Basic & Clinical Pharmacology, 3(5), 774–780. Retrieved from https://www.ijbcp.com/index.php/ijbcp/article/view/1084

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Original Research Articles